| Basic data |
|---|
| OCA1. Surname awarded to the assessed person | Ševčíková |
|---|
| OCA2. Name awarded to the assessed person | Zuzana |
|---|
| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
|---|
| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6009 |
|---|
| OCA5. Area of assessment | habilitation and inauguration procedure – general veterinary medicine |
|---|
| OCA6. Category of the research/artistic/other output | scientific output |
|---|
| OCA7. Year of publication of the research/artistic/other output | 2023 |
|---|
| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | ID 1053156 |
|---|
| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=E84B3E3EDA810AC00D6D568372D1 |
|---|
| Characteristics of the output that is not registered in CRPA or CRAA |
|---|
| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | |
|---|
| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | KARAFFOVÁ, Viera - TELEKY, Jana - PINTARIČ, Máša - LANGERHOLC, Tomaž - MUDROŇOVÁ, Dagmar - HUDEC, Erik - ŠEVČÍKOVÁ, Zuzana. Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) improves immunological profile of the non-carcinogenic porcine-derived enterocytes. In Life. ISSN 2075-1729, 2023, vol. 13, no. 5, art. no. 1090, p. [1-14]. (2022: 3.200 - IF, 3.200 - IF 5y, 46 - H-index, 0.634 - SJR, Q2 - SJR Best Q, Q2 - JCR Best Q) |
|---|
| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | |
|---|
| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.mdpi.com/2075-1729/13/5/1090 |
|---|
| OCA14. Characteristics of the author's contribution | Management and implementation of trials and sampling collections |
|---|
| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The results showed that the incubation of the CLAB cell line with probiotic bacteria significantly increased the metabolic activity of the cells and subsequently increased the cell viability of the CLAB cell line markedly at a concentration of 109 CFU/mL after both exposure times. The data obtained in this study indicate that the response of CLAB cells to Lactobacillus reuteri B1/1 exposure varies depending on its concentration and duration of exposure. |
|---|
| Other data |
|---|
| OCA16. Annotation of the output in English | In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri (L. reuteri), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 × 107 and 1 × 109 CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1β, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). CLAB cells were cultured in a 12-well cell culture plate at a concentration of 4 × 105 cells/well in DMEM medium in a controlled humidified atmosphere for 48 h. A 1 mL volume of each probiotic bacterial suspension was added to the CLAB cells. Plates were incubated for 2 h and 4 h. Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation. |
|---|
| OCA17. List of maximum 5 most significant citations corresponding to the output | |
|---|
| OCA18. Characteristics of the output's impact on socio-economic practice | The extensive breeding of farm animals and the demands of consumers on the quality of animal products place emphasis on breeders and producers in the direction of improving animal health. In this context, in the last decade, the attention of the public and scientists has been focused on gaining knowledge about the use of probiotic bacteria and their products in animal health and diseases. |
|---|
| OCA19. Characteristics of the output and related activities' impact on the educational process | The physiology, pathology and immune system of the digestive tract, as well as its ability to adapt to various stimuli, constitute a complex and very extensive field of knowledge in veterinary medicine. The importance of a study on the impact of probiotics on GIT, overall performance and animal health is steadily increasing in relation to the provision of a one- health strategy. |
|---|
