LAUKOVÁ, Andrea [45 %] - MICENKOVÁ, Lenka [15 %] - KUBAŠOVÁ, Ivana [5 %] - BINO, Eva [5 %] - KANDRIČÁKOVÁ, Anna [5 %] - PLACHÁ, Iveta [5 %] - ŠTRKOLCOVÁ, Gabriela [5 %] - GÁLIK, Branislav [5 %] - KOVÁČIK, Anton [3 %] - HALO, Marko [2 %] - POGÁNY SIMONOVÁ, Monika [5 %]. Microbiota, phagocytic activity, biochemical parameters and parasite control in horses with application of autochthonous, bacteriocin-producing, probiotic strain Enterococcus faecium EF 412. In Probiotics and Antimicrobial Proteins. ISSN 1867-1306, 2023, vol. 15, no. 1, p. 139-148. (2022: 4.900 - IF, 5.400 - IF 5y, 44 - H-index, 0.804 - SJR, Q2 - SJR Best Q, Q1 - JCR Best Q). Dostupné na internete: .

Basic data
OCA1. Surname awarded to the assessed person	Štrkolcová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/26622
OCA5. Area of assessment	habilitation and inauguration procedure – food hygiene and the environment
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2023
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	DOI10.1007/s12602-022-09918-4
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildIN7QE&sid=D8DE6E8D222079A628EB391F7EDF&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://pubmed.ncbi.nlm.nih.gov/35119612/
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	LAUKOVÁ, Andrea [45 %] - MICENKOVÁ, Lenka [15 %] - KUBAŠOVÁ, Ivana [5 %] - BINO, Eva [5 %] - KANDRIČÁKOVÁ, Anna [5 %] - PLACHÁ, Iveta [5 %] - ŠTRKOLCOVÁ, Gabriela [5 %] - GÁLIK, Branislav [5 %] - KOVÁČIK, Anton [3 %] - HALO, Marko [2 %] - POGÁNY SIMONOVÁ, Monika [5 %]. Microbiota, phagocytic activity, biochemical parameters and parasite control in horses with application of autochthonous, bacteriocin-producing, probiotic strain Enterococcus faecium EF 412. In Probiotics and Antimicrobial Proteins. ISSN 1867-1306, 2023, vol. 15, no. 1, p. 139-148. (2022: 4.900 - IF, 5.400 - IF 5y, 44 - H-index, 0.804 - SJR, Q2 - SJR Best Q, Q1 - JCR Best Q). Dostupné na internete: .
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://pubmed.ncbi.nlm.nih.gov/35119612/
OCA14. Characteristics of the author's contribution	Co-author; practical parts
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The study focused on evaluating the effects of an autochthonous, bacteriocin-producing fecal strain Enterococcus faecium EF 412 on the health and gut microbiota of horses. Twelve clinically healthy Slovak Warmblood horses were included in the experiment, receiving a daily dose of 1 g of lyophilized EF 412 for 21 days. Samples were collected at three time points: at the beginning, after 21 days of application, and 14 days after cessation. The results showed successful colonization of the gastrointestinal tract by EF 412. No Strongylus spp. nematode eggs or Eimeria spp. oocysts were found in the feces after application, indicating a potential antiparasitic effect. The rest of the gut microbiota was not negatively affected. Next-generation sequencing revealed that the dominant phyla were Bacteroidetes and Firmicutes, while at the family level, Bacteroidales BS11, S24-7, and Lentisphaerae were most prevalent. An increase in phagocytic activity was observed, along with changes in biochemical parameters—specifically, a decrease in total protein, cholesterol, and triglycerides during EF 412 administration. The autochthonous strain E. faecium EF 412 demonstrated promising application potential as a probiotic for horses, with positive effects on gut health and immune response, without disrupting microbial balance.
Other data
OCA16. Annotation of the output in English	The autochthonous fecal strain Enterococcus faecium EF 412 successfully colonized the gastrointestinal tract of horses and increased the total counts of enterococci and lactic acid bacteria (LAB). It induced shifts in the horse gut microbiota, as demonstrated by both standard microbiological methods and next-generation sequencing at the phylum and family levels. Although the phagocytic activity (PA) in the blood was not significantly increased, there was a tendency to stimulate an unspecified immune parameter. Biochemical parameters remained within the physiological range throughout the study. Notably, no nematode eggs (Strongylus spp.) or Eimeria spp. oocysts were detected in the horses after EF 412 administration. These results suggest that the autochthonous fecal strain E. faecium EF 412 has promising potential for application in horse health management.
OCA17. List of maximum 5 most significant citations corresponding to the output	Nogacka AM, García A, G de Los Reyes-Gavilán C, Arboleya S, Gueimonde M. In vitro assessment of horse-isolated strains of Lactobacillus acidophilus and Ligilactobacillus equi species for fecal microbiota modulation in horses. J Equine Vet Sci. 2025 Feb;145:105341. doi: 10.1016/j.jevs.2024.105341. Epub 2024 Dec 30. PMID: 39742928. Loo, K.-Y., Thong, J. Y. H., Tan, L. T.-H., Letchumanan, V., Chan, K.-G., Lee, L.-H., & Law, J. W.-F. (2024). A Current Overview of Next-Generation Probiotics and Their Prospects in Health and Disease Management . Progress In Microbes & Molecular Biology, 7(1). https://doi.org/10.36877/pmmb.a0000457 Ericsson, A.C. A Comprehensive Review of the Equine Gut Microbiome in Health and Disease. Vet. Sci. 2026, 13, 659. https://doi.org/10.3390/vetsci13070659 Peng Y, Shen X. Supplementation of a Dietary Probiotics Blend can Enhance Growth Performance in Guiqian Semi-Fine Wool Sheep by Modulating Rumen Microbiota and Immune Function. Probiotics Antimicrob Proteins. 2026 Apr;18(3):4569-4580. doi: 10.1007/s12602-025-10757-2. Epub 2025 Oct 4. PMID: 41045441. Lauková, A.; Ščerbová, J.; Focková, V.; Stojanov, I.; Pogány Simonová, M.; Prodanov-Radulović, J. Assessing Virulence Factor Genes in Pig-Derived Escherichia coli from the Region of Vojvodina Treated with Postbiotic Substance and Herbal Essential Oils. Pathogens 2026, 15, 215. https://doi.org/10.3390/pathogens15020215
OCA18. Characteristics of the output's impact on socio-economic practice	Findings related to the autochthonous strain Enterococcus faecium EF 412 provide valuable knowledge in microbiology, immunology, and veterinary sciences, thereby enriching the educational content for students in these fields. Studying the colonization of the equine gastrointestinal tract, changes in microbiota, and immune responses allows students to better understand the application of probiotics and host-microorganism interactions in large animals. This research supports the integration of modern techniques into educational programs, improving students’ practical skills and their knowledge of advanced molecular methods. At the same time, it encourages interdisciplinary education by combining microbiology, parasitology, biochemistry, and animal health, fostering critical thinking and research skills necessary for future professionals in veterinary and animal sciences.
OCA19. Characteristics of the output and related activities' impact on the educational process	Findings related to the autochthonous strain Enterococcus faecium EF 412 provide valuable knowledge in microbiology, immunology, and veterinary sciences, thereby enriching the educational content for students in these fields. Studying the colonization of the equine gastrointestinal tract, changes in microbiota, and immune responses allows students to better understand the application of probiotics and host-microorganism interactions in large animals. This research supports the integration of modern techniques into educational programs, improving students’ practical skills and their knowledge of advanced molecular methods. At the same time, it encourages interdisciplinary education by combining microbiology, parasitology, biochemistry, and animal health, fostering critical thinking and research skills necessary for future professionals in veterinary and animal sciences.