KADUKOVÁ, Michaela [29 %] - SCHREIBEROVÁ, Andrea [28 %] - MUDROŇ, Pavol [6 %] - TÓTHOVÁ, Csilla [6 %] - GOMULEC, Pavel [3 %] - ŠTRKOLCOVÁ, Gabriela [28 %]. Cryptosporidium infections in neonatal calves on a dairy farm. In Microorganisms. ISSN 2076-2607, 2024, vol. 12, no. 7, art. no. 1416, p. [1-11]. (2022: 4.5 - IF, 4.8 - IF 5y, 66 - H-index, 0.909 - SJR, Q2 - SJR Best Q, Q2 - JCR Best Q). Dostupné na internete: .

Basic data
OCA1. Surname awarded to the assessed person	Štrkolcová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/26622
OCA5. Area of assessment	habilitation and inauguration procedure – food hygiene and the environment
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	10.3390/microorganisms12071416
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=20AC8DF1557D29D7190CC74B25DB
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.semanticscholar.org/paper/Cryptosporidium-Infections-in-Neonatal-Calves-on-a-Kadukov%C3%A1-Schreiberov%C3%A1/93a82c7631870e4e4f745bfe7dba4200bea5ab35
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	KADUKOVÁ, Michaela [29 %] - SCHREIBEROVÁ, Andrea [28 %] - MUDROŇ, Pavol [6 %] - TÓTHOVÁ, Csilla [6 %] - GOMULEC, Pavel [3 %] - ŠTRKOLCOVÁ, Gabriela [28 %]. Cryptosporidium infections in neonatal calves on a dairy farm. In Microorganisms. ISSN 2076-2607, 2024, vol. 12, no. 7, art. no. 1416, p. [1-11]. (2022: 4.5 - IF, 4.8 - IF 5y, 66 - H-index, 0.909 - SJR, Q2 - SJR Best Q, Q2 - JCR Best Q). Dostupné na internete: .
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.semanticscholar.org/paper/Cryptosporidium-Infections-in-Neonatal-Calves-on-a-Kadukov%C3%A1-Schreiberov%C3%A1/93a82c7631870e4e4f745bfe7dba4200bea5ab35
OCA14. Characteristics of the author's contribution	co-author - bearer of ideas in the text
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	This research output was developed as part of scientific creative activity focused on the molecular identification of the protozoan parasite Cryptosporidium spp. in calves during early developmental stages. The study was conducted on a dairy farm in eastern Slovakia and included 25 Holstein and Holstein-crossbred calves, monitored from birth to the fifth week of life. Fresh fecal samples were collected weekly—except during the fourth week—and analyzed using Ziehl–Neelsen staining and a screening ELISA test for the detection of cryptosporidial antigen. The highest incidence of infection was recorded in the second week of life—91.6% of samples tested positive by ELISA and 62.5% by Ziehl–Neelsen staining. Positive findings were further molecularly characterized using PCR and sequence analysis of the 18S rRNA and gp60 genes. The analysis revealed the presence of two Cryptosporidium species: C. parvum and C. ryanae, with the zoonotic species C. parvum identified in 25 out of 29 isolates. Genotyping based on the gp60 locus confirmed the presence of the IIaA17G1R1 subtype. The results highlight the role of calves as significant reservoirs of zoonotic C. parvum, underlining the public health relevance of exposure to livestock, their feces, and manure handling as potential sources of infection not only for animals but also for humans.
Other data
OCA16. Annotation of the output in English	he study investigates the occurrence and molecular characterization of Cryptosporidium spp. in dairy calves during the first five weeks of life on a farm in Eastern Slovakia. Twenty-five Holstein and Holstein-cross calves were monitored, with fecal samples examined using Ziehl–Neelsen staining and an ELISA coproantigen test. The highest prevalence of infection was recorded during the second week of life, when ELISA detected Cryptosporidium antigen in 91.6% of calves, while Ziehl–Neelsen staining indicated a prevalence of 62.5%. Molecular analysis of positive isolates based on the 18S rRNA and gp60 genes identified two species, Cryptosporidium parvum and Cryptosporidium ryanae. Of 29 molecularly characterized isolates, 25 were identified as C. parvum, with the gp60 subtype IIaA17G1R1 detected. The findings demonstrate the high susceptibility of young calves to cryptosporidiosis and confirm their important role as reservoirs of zoonotic C. parvum. The study also highlights the public health significance of cryptosporidiosis, particularly the potential risk of transmission to humans through direct contact with infected livestock, feces, and contaminated manure.
OCA17. List of maximum 5 most significant citations corresponding to the output	Baazizi R, Taibi M, Senouci N, Baroudi D, Khodja S, Belala R, Khelef D, Mimoune N. Prevalence and risk factors of Cryptosporidium spp. and Giardia infestation in cattle and in broiler chickens in Algeria. Vet Med (Praha). 2025 Feb 24;70(2):68-76. doi: 10.17221/82/2024-VETMED. PMID: 40115530; PMCID: PMC11922057. María Cristina Del Río, Sergio Martín, Joaquín Quílez, José Manuel Molina, Otilia Ferrer, José Adrián Molina, Adrián Melián & Antonio Ruiz. (2025) Molecular Epidemiology of Cryptosporidiosis on Lamb and Goat Kid Farms in Gran Canaria, Canary Islands (Spain). Microorganisms 13:3, pages 644. Zolova, A., Keidāne, D., & Zolovs, M. (2024). A seven-year study on the prevalence and intensity of Cryptosporidium spp. infections in dairy cattle in Latvia: regional and age-related variations. Acta Biologica Universitatis Daugavpiliensis, 2024(2), 239-246. https://doi.org/10.59893/abud.24(2).008 Abd El-Aziz, T.H., Ata, E.B., Helal, M.A. et al. Seroprevalence and risk factors associated with calves’ cryptosporidiosis in Egypt. BMC Vet Res 22, 125 (2026). https://doi.org/10.1186/s12917-025-05216-7 Peng, X., Wang, X., Jian, J. et al. Investigation of Cryptosporidium spp. and Enterocytozoon bieneusi in free-ranged livestock on the southeastern Qinghai–Xizang Plateau, China. BMC Infect Dis 25, 356 (2025). https://doi.org/10.1186/s12879-025-10737-5
OCA18. Characteristics of the output's impact on socio-economic practice	The results of the study have significant potential for application in veterinary, livestock-production, and public-health practice in Slovakia. The identification of Cryptosporidium species and genotypes in calves during the early stages of life provides important information on the occurrence and dynamics of cryptosporidiosis in cattle herds. The demonstrated high prevalence of infection, particularly during the second week of life, enables preventive, diagnostic, and zoohygienic measures to be more effectively targeted at the period associated with the highest risk of infection. From an economic perspective the findings may contribute to reducing losses associated with diarrhoeal diseases, impaired health, reduced growth performance, and increased costs of treatment and management of young animals. The implementation of effective preventive and hygienic measures on farms may improve calf health and welfare, reduce infection pressure within the farm environment, and enhance production efficiency. The identification and molecular characterization of the zoonotic species Cryptosporidium parvum also emphasize the public-health relevance of the findings. Calves may represent an important reservoir of infection for humans, particularly farm workers, veterinarians, and other individuals exposed to livestock, animal feces, or farm manure. The results therefore support the One Health approach, integrating animal, human, and environmental health. The knowledge obtained from this study may be applied in the development of preventive programmes, improvement of farm biosecurity measures, and epidemiological surveillance of cryptosporidiosis in Slovakia.
OCA19. Characteristics of the output and related activities' impact on the educational process	The results of this study and related activities have a significant impact on the educational process in the field of human as well as veterinary medicine and parasitology. They provide valuable material for expanding knowledge about zoonotic diseases and parasitic infections that can be transmitted between animals and humans. The findings offer practical information on the effectiveness of various antiparasitic therapies, which is essential for proper diagnosis and treatment of these diseases in clinical practice. The data obtained can be used as a foundation for educational materials, lectures, and seminars that cover not only theoretical knowledge about zoonoses but also practical aspects of diagnosing and treating parasitic diseases. The study also highlights the importance of accurate parasitological examination and communication between veterinarians and pet owners, which is crucial for preventing misdiagnosis and inappropriate treatment.