MUDROŇOVÁ, Dagmar - KARAFFOVÁ, Viera - SEMJON, Boris - NAĎ, Pavel - KOŠČOVÁ, Jana - BARTKOVSKÝ, Martin - MAKIŠ, Andrej - BUJŇÁK, Lukáš - NAGY, Jozef - MOJŽIŠOVÁ, Jana - MARCINČÁK, Slavomír. Effects of dietary supplementation of humic substances on production parameters, immune status and gut microbiota of laying hens. In Agriculture. ISSN 2077-0472, 2021, vol. 11, no. 8, art. no. 744,p. [1-14].

Basic data
OCA1. Surname awarded to the assessed person	Mudroňová
OCA2. Name awarded to the assessed person	Dagmar
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6094
OCA5. Area of assessment	habilitation and inauguration procedure – microbiology and immunology
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	414994
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=BD8873FEBC965481A59310BA7E
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000688745800001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	MUDROŇOVÁ, Dagmar - KARAFFOVÁ, Viera - SEMJON, Boris - NAĎ, Pavel - KOŠČOVÁ, Jana - BARTKOVSKÝ, Martin - MAKIŠ, Andrej - BUJŇÁK, Lukáš - NAGY, Jozef - MOJŽIŠOVÁ, Jana - MARCINČÁK, Slavomír. Effects of dietary supplementation of humic substances on production parameters, immune status and gut microbiota of laying hens. In Agriculture. ISSN 2077-0472, 2021, vol. 11, no. 8, art. no. 744,p. [1-14].
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2077-0472/11/8/744
OCA14. Characteristics of the author's contribution	The author is one of the bearers of the main idea of the output and at the same time the implementer and creator of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	In this study we found out that the addition of 0.5% HS to laying hen feed had a positive effect on laying rate, daily egg mass, egg weight, feed conversion, and eggshell quality and also had an immunostimulatory effect manifested by the increasing activity of the phagocytes and the B cell immune response. On the other hand, there was an observed increase in the number of enterobacteria in the intestinal contents, and the proportion of T lymphocytes in the blood was significantly reduced. The presented results confirm that HS can be used for the improvement of egg production and quality and for activation of phagocytosis and specific antibody immunity, but their influence on the intestinal microbiota will need to be further studied with respect to a wider range of microbial species inhabiting the digestive tract of laying hens.
Other data
OCA16. Annotation of the output in English	Despite the fact that humic substances (HS) have been frequently studied in relation to their effects on livestock health, studies on their influence on egg production and quality, immunity, and intestinal microbiota of laying hens are limited. In this study, the influence of 0.5% HS supplementation on the specific production parameters of eggshell mineral quality, immune parameters (relative expression of IgA, IGF-2, MUC-2 gene in cecum; phagocytic activity, percentage of selected lymphocyte subpopulations in the peripheral blood), and number of lactic acid bacteria and enterobacteria in the intestinal contents was tested. The addition of 0.5% HS to the laying hen feed had a positive effect on egg laying rate, daily egg mass, egg weight, feed conversion and eggshell quality and also had an immunostimulatory effect manifested by increased phagocyte activity and B cell response. Concurrently, an increase in the number of enterobacteria in the intestinal contents and a decrease in the proportion of T lymphocytes (p < 0.05) was observed, which can be considered as a negative effect of HS. The results confirmed that HS can be used for the improvement of egg production and targeted immunostimulation, but their effect on the intestinal microbiota and T lymphocytes should be studied.
OCA17. List of maximum 5 most significant citations corresponding to the output	1. Santos, T.C.d.; Silva, H.P.; Lima, K.R.; Salvador, M.L.N.; Cândido, G.d.S.; Pimenta, L.C.J.P.; Bertolini, N.O.; Ribeiro, L.B.; Fagundes, F.G.; Orlando, D.R.; et al. Humic Acid Derived from Vermicompost Improves Bone Mineral Content and Alters Oxidative Stress Markers in Ovariectomized Mice. Biomedicines 2025, 13, 495. https://doi.org/10.3390/biomedicines13020495 2. Tavares HG, Orlando PR, de Souza Pereira RR, Magalhães CODE, Silva G, Dos Santos Nunes Ferreira A, Garcia BCC, Lima KR, Vieira ER, Dobbss LB, Dias-Peixoto MF, Machado ART, Pereira LJ, Andrade EF. Humic acid from vermicompost effectively regulates the redox status and mitigates the progression of experimental periodontitis. Naunyn Schmiedebergs Arch Pharmacol. 2025 Jan 10. doi: 10.1007/s00210-024-03747-8. 3. Sun M, Shi Y, Lei B, Zhang W, Feng J, Ge S, Yuan W, Zhao K. A pH-triggered self-releasing humic acid hydrogel loaded with porcine interferon α/γ achieves anti-pseudorabies virus effects by oral administration. Vet Res. 2024 Nov 20;55(1):153. doi: 10.1186/s13567-024-01411-w. 4. Orlando PR, Tavares HG, de Souza Pereira RR, Silva G, do Carmo Lima Carvalho J, Machado ART, Dobbss LB, Peixoto MFD, Pereira LJ, Andrade EF. Humic Acid Derived from Agricultural Biomass Mitigates Alveolar Bone Loss and Modulates Systemic Inflammatory Cytokines in Rats with Periodontitis. Curr Top Med Chem. 2025;25(5):645-656. doi: 10.2174/0115680266302476240510115556. 5. Huang J, Xu P, Shao M, Wei B, Zhang C, Zhang J. Humic acids alleviate dextran sulfate sodium-induced colitis by positively modulating gut microbiota. Front Microbiol. 2023 Apr 12;14:1147110. doi: 10.3389/fmicb.2023.1147110.
OCA18. Characteristics of the output's impact on socio-economic practice	The results confirm the positive effect of humic substances on egg quality and production, as well as on certain components of the immune system, which has a beneficial impact on the health and resilience of laying hens. Based on the obtained results, the tested preparation at the given dose can be recommended to poultry farmers and introduced into practice.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output brings new knowledge about the immune response of poultry after the application of humic substances. As this area is still little studied, this knowledge will be used for further education of students in the 3rd level of university studies in the program Microbiology and Immunology. A doctoral student (MVDr. Andrej Makiš, PhD.) also participated in the implementation of the experiment, who acquired new practical skills, mastered new methodologies, and used the results in his dissertation.