BUJŇÁK, Lukáš - HREŠKO ŠAMUDOVSKÁ, Alena - MUDROŇOVÁ, Dagmar - NAĎ, Pavel - MARCINČÁK, Slavomír - MASKAĽOVÁ, Iveta - HARČÁROVÁ, Michaela - KARAFFOVÁ, Viera - BARTKOVSKÝ, Martin. The effect of dietary humic substances on cellular immunity and blood characteristics in piglets. In Agriculture. ISSN 2077-0472, 2023, vol. 13, no. 3, art. no. 636, p. [1-12]

Basic data
OCA1. Surname awarded to the assessed person	Mudroňová
OCA2. Name awarded to the assessed person	Dagmar
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6094
OCA5. Area of assessment	habilitation and inauguration procedure – microbiology and immunology
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2023
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	1042465
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=6E72E145B6DED42F49C5E40625D6
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000953705000001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	BUJŇÁK, Lukáš - HREŠKO ŠAMUDOVSKÁ, Alena - MUDROŇOVÁ, Dagmar - NAĎ, Pavel - MARCINČÁK, Slavomír - MASKAĽOVÁ, Iveta - HARČÁROVÁ, Michaela - KARAFFOVÁ, Viera - BARTKOVSKÝ, Martin. The effect of dietary humic substances on cellular immunity and blood characteristics in piglets. In Agriculture. ISSN 2077-0472, 2023, vol. 13, no. 3, art. no. 636, p. [1-12]
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2077-0472/13/3/636
OCA14. Characteristics of the author's contribution	The author is one of the bearers of the main idea of the output and at the same time the implementer and creator of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The effect of humic substances on the cellular immune response in weaned pigs has not been sufficiently investigated. In this experiment we found out that a diet supplementation with 0.5% humic substances could have a stimulatory effect on some immune cells in piglets. There was a significant increase in the proportion of CD4+CD8- lymphocytes in the blood. Supplementation with humic substances increased serum ALP. However, these values were still within the reference range. The results showed that even such a low concentration of HS can positively affect cellular immunity in piglets. Due to the fact, that the concentration of humic substances we chose had a significant effect only on some indicators of cellular immunity, further studies will be necessary to choose an appropriate concentration and confirm the stimulating effect on immunity in this category of animals.
Other data
OCA16. Annotation of the output in English	This study’s objective was to determine the impact of dietary humic substances on immune response and blood profiles in piglets. A total of 24 crossbred piglets (Slovakian White × Landrace; 35 days old; average body weight of 11.67 kg) were allotted to two dietary groups with (experimental; 5 g·kg−1) or without (control; 0 g·kg−1) natural humic substances supplementation. In this study, we observed a significant increase of the proportion of CD4+CD8- lymphocytes (p < 0.001) in the experimental group. The results also showed a tendency for an increase of the phagocytic activity and the engulfing capacity of phagocytes and the numbers of the other monitored lymphocyte subpopulations (CD3+, CD21+, CD4-D8+, CD4+CD8+, CD4+CD25+) in piglets in the experimental group compared to the control group. Supplementation of humic substances increased serum alkaline phosphatase compared to the control group (p < 0.05). Other monitored blood parameters were not significantly affected by dietary treatment. It concluded that inclusion of humic substances in the diet of piglets could have a stimulating effect on cellular immunity, without a negative effect on haematological and biochemical parameters.
OCA17. List of maximum 5 most significant citations corresponding to the output	Roulia, M. Sustainable Utilization of Humic Substances and Organic Waste in Green Agriculture. Agriculture 2024, 14, 115. https://doi.org/10.3390/agriculture1401011 Edmonds MS, Weber TE. Efficacy of water application of a humic substance, butyric acid, vitamins C, D, and E and/or electrolytes on performance and mortality in health-challenged nursery pigs¹. Transl Anim Sci. 2023 Oct 2;7(1):txad115. doi: 10.1093/tas/txad115. Maguey-González JA, Nava-Ramírez MJ, Gómez-Rosales S, Ángeles ML, Solís-Cruz B, Hernández-Patlán D, Merino-Guzmán R, Hernandez-Velasco X, Hernández-Ramírez JO, Loeza I, Senas-Cuesta R, Latorre JD, Vázquez-Durán A, Du X, Méndez-Albores A, Hargis BM, Téllez-Isaías G. Evaluation of the efficacy of humic acids to counteract the toxic effects of aflatoxin B1 in turkey poults. Front Vet Sci. 2023 Oct 10;10:1276754. doi: 10.3389/fvets.2023.1276754. Erratum in: Front Vet Sci. 2023 Dec 12;10:1346080. doi: 10.3389/fvets.2023.1346080. Hriciková S, Kožárová I, Koréneková B, Marcinčák S. The Effect of the Supplementation of Humic Substances and Fermented Products in the Feed on the Content of Salinomycin Residues in Poultry Tissues. Foods. 2023 Dec 24;13(1):68. doi: 10.3390/foods13010068. Zykova, M.V.; Trofimova, E.S.; Azarkina, L.A.; Lasukova, T.V.; Mihalyov, D.A.; Drygunova, L.A.; Danilets, M.G.; Ligacheva, A.A.; Tsupko, A.V.; Bashirov, S.R.; et al. Pharmacological Effects of Humic Substances and Their Signaling Mechanisms. Molecules 2026, 31, 114. https://doi.org/10.3390/molecules31010114
OCA18. Characteristics of the output's impact on socio-economic practice	Since the influence of humic substances on the immunity of weaned pigs is not sufficiently investigated, in this pilot study we focused on monitoring the parameters of cellular immunity in this category of animals. The results revealed an immunostimulating effect of 0.5% HS addition, without disturbing the hematological and biochemical blood profile of pigs, which is a prerequisite for their possible use in breeding practice.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output brings new knowledge about the immune response of weaned pigs after the application of humic substances. As this area is still little studied, this knowledge will be used for further education of students in the 3rd level of university studies in the program Microbiology and Immunology.