KARAFFOVÁ, Viera - TELEKY, Jana - PINTARIČ, Máša - LANGERHOLC, Tomaž - MUDROŇOVÁ, Dagmar - HUDEC, Erik - ŠEVČÍKOVÁ, Zuzana. Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) improves immunological profile of the non-carcinogenic porcine-derived enterocytes. In Life. ISSN 2075-1729, 2023, vol. 13, no. 5, art. no. 1090, p. [1-14]

Basic data
OCA1. Surname awarded to the assessed person	Mudroňová
OCA2. Name awarded to the assessed person	Dagmar
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6094
OCA5. Area of assessment	habilitation and inauguration procedure – microbiology and immunology
OCA6. Category of the research/artistic/other output
OCA7. Year of publication of the research/artistic/other output	2023
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	1053156
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=E84B3E3EDA810AC00D6D568372D1
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000998062900001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	KARAFFOVÁ, Viera - TELEKY, Jana - PINTARIČ, Máša - LANGERHOLC, Tomaž - MUDROŇOVÁ, Dagmar - HUDEC, Erik - ŠEVČÍKOVÁ, Zuzana. Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) improves immunological profile of the non-carcinogenic porcine-derived enterocytes. In Life. ISSN 2075-1729, 2023, vol. 13, no. 5, art. no. 1090, p. [1-14]
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2075-1729/13/5/1090
OCA14. Characteristics of the author's contribution	The author is one of the bearers of the main idea of the output, probiotic strain provider and at the same time the creator of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	In this study, we focused on revealing the mechanism of action of the probiotic strain L. reuteri B1/1, which was isolated and characterized at the Department of Microbiology and Immunology, UVLF in Košice. In our previous studies, the B1/1 strain was tested in vitro on mononuclear leukocytes from the peripheral blood of chickens, where it effectively modulated the gene expression of pro-inflammatory cytokines and the representation of T lymphocyte subpopulations. Its effect was also confirmed in an in vivo experiment on broiler chickens infected with Campylobacter jejuni. Our results of this experiment revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both lumican and olfactomedin 4 in an intact CLAB cell line after 4 h of incubation. Therefore, we hypothesize that L. reuteri B1/1 may also participate in strengthening the integrity of the intestinal mucosa.
Other data
OCA16. Annotation of the output in English	In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri (L. reuteri), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 × 107 and 1 × 109 CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1β, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation.
OCA17. List of maximum 5 most significant citations corresponding to the output	Kiššová Z, Štofilová J, Mudroňová D, Karaffová V. Newly Isolated Limosilactobacillus reuteri B1/1 Modulates the Expression of Cytokines and Antimicrobial Proteins in a Porcine ex Vivo Model. Front Biosci (Landmark Ed). 2024 May 10;29(5):180. doi: 10.31083/j.fbl2905180. Kiššová Z, Mátis G, Mackei M, Tráj P, Márton RA, Horváth DG, Tóthová C, Mudroňová D, Karaffová V. Research note: utilizing a novel chicken ileal explant model to assess the efficacy of probiotic Limosilactobacillus reuteri CCM 9425 against Salmonella Enteritidis infection. Poult Sci. 2025 Apr;104(4):104909. doi: 10.1016/j.psj.2025.104909.
OCA18. Characteristics of the output's impact on socio-economic practice	The results presented in this work, where we were able to reveal the positive effect of the probiotic strain L. reuteri B1/1 on the integrity of the intestinal mucosa, were the basis for further research, which we carried out on a unique model of poultry intestinal explants, where we subsequently clarified the immunomodulatory effects of the B1/1 strain. These experiments are a necessary prerequisite for its future use in the poultry industry.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output brings new knowledge about the mechanism of action of beneficial bacteria and their influence on the integrity of the intestinal barrier. The work was created on the basis of international cooperation with colleagues from the University of Maribor, where Erasmus stays were implemented. The expansion of the methodological background and the new knowledge gained are used for further education of students in the 3rd level of university studies in the programs: Microbiology and Immunology, and Infectious Diseases of Animals.