| Basic data |
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| OCA1. Surname awarded to the assessed person | Mudroňová |
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| OCA2. Name awarded to the assessed person | Dagmar |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6094 |
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| OCA5. Area of assessment | habilitation and inauguration procedure – microbiology and immunology |
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| OCA6. Category of the research/artistic/other output | |
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| OCA7. Year of publication of the research/artistic/other output | 2023 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 1053867 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=E84B3E3EDA810AC0046E578372D1 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://www.webofscience.com/wos/woscc/full-record/WOS:001013876400001 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | JURČACKOVÁ, Zuzana - CIGLANOVÁ, Denisa - MUDROŇOVÁ, Dagmar - TUMOVÁ, Lenka - BÁRCENAS-PÉREZ, Daniela - KOPECKÝ, Jiří - KOŠČOVÁ, Jana - CHEEL, José - HRČKOVÁ, Gabriela. Astaxanthin extract from Haematococcus pluvialis and its fractions of Astaxanthin mono- and diesters obtained by CCC show differential antioxidant and cytoprotective effects on naïve-mouse spleen cells. In Antioxidants. ISSN 2076-3921, 2023, vol. 12, no. 6, art. no. 1144, p. [1-25] |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.mdpi.com/2076-3921/12/6/1144 |
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| OCA14. Characteristics of the author's contribution | The author is implementer and at the same time the creator of the output. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | In our study, we investigated the effects of Haematococcus pluvialis extract corresponding to the total carotenoid astaxanthin-rich fraction and astaxanthin monoesters and diesters obtained by countercurrent chromatography (CCC) on selected physiological functions, antioxidant enzyme gene expression, and scavenging capacity for cell-producing reactive oxygen/nitrogen species (ROS/NO) in unstimulated spleen cells from healthy mice cultivated in vitro. Based on our results, we demonstrated that differential activities of ME and DE contribute to the final antioxidant and cytoprotective effects of astaxanthin extract, which is beneficial in preventing a wide range of ROS-induced adverse effects, with DE being more effective. In addition, the selection of physioxia-like conditions for pharmacological research is highlighted. |
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| Other data |
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| OCA16. Annotation of the output in English | Carotenoids are abundant lipid-soluble phytochemicals often used as dietary supplements to protect against oxidative stress-related diseases. Astaxanthin, a xanthophyll carotenoid, is a potent antioxidant with multiple beneficial effects on cellular functions and signaling. In this study, spleen cells from healthy Balb/c mice were treated with an astaxanthin-rich extract (EXT) from Haematococcus pluvialis and its monoester-rich (ME) and diester-rich (DE) fractions obtained by countercurrent chromatography. After 24 h under standard culture conditions, untreated splenocyte viability dropped to ~75%, correlating with decreased mitochondrial membrane potential, early apoptosis in ~59% of cells, and reduced ROS production, suggesting hyperoxia-related functional decline. Co-cultivation with EXT, ME, and DE (up to 10 µg/mL) restored or stimulated cell functions, with effectiveness ranked as EXT > DE > ME, indicating esterification enhances bioavailability in vitro. ROS and H₂O₂ levels mirrored mRNA expression of Nrf2, SOD1, catalase, and glutathione peroxidase 1, while inversely correlating with iNOS-mediated NO production. At 40 µg/mL, all samples were detrimental, likely due to excessive scavenging of reactive species needed for normal signaling. The study shows that ME and DE differentially contribute to the extract’s antioxidant and cytoprotective effects, with DE being more effective, and emphasizes the importance of physioxia-like conditions in research. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | Lamya Al Fuhaid, Gordon B. Wellman, Najeh Kharbatia, Andreia S.F. Farinha, Johannes S. Vrouwenvelder, Rob Verpoorte, Young Hae Choi, Geert-Jan Witkamp, Kyle J. Lauersen, Luca Fortunato, Green extraction of pigment from astaxanthin-producing algae using natural deep eutectic solvents, Algal Research, Volume 82, 2024, 103668, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2024.103668.
Fu S, Li J, You J, Liu S, Dong Q, Fu Y, Luo R, Sun Y, Tian X, Liu W, Zhang J, Ding Y, Zhang Y, Wang W, Guo L, Qiu Y. Baicalin attenuates PD-1/PD-L1 axis-induced immunosuppression in piglets challenged with Glaesserella parasuis by inhibiting the PI3K/Akt/mTOR and RAS/MEK/ERK signalling pathways. Vet Res. 2024 Jul 29;55(1):95. doi: 10.1186/s13567-024-01355-1. Erratum in: Vet Res. 2024 Sep 30;55(1):127. doi: 10.1186/s13567-024-01375-x.
Makay, K.; Grewe, C. Towards Microalgal Biorefinery: Multiproduct Fractionation of Phaeodactylum tricornutum by Liquid–Liquid Techniques. Mar. Drugs 2026, 24, 242. https://doi.org/10.3390/md24070242
Chen, T.; Zhu, X.; Liao, Q. Resolving the “Thick-Wall Challenge” in Haematococcus pluvialis: From Metabolic Reprogramming to Clinical Translation. Microorganisms 2026, 14, 253. https://doi.org/10.3390/microorganisms14010253 |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The results of this work, which is of a basic research nature, are the basis for further research and subsequent applicability of these natural antioxidants in practice, both in veterinary and human medicine. The results obtained in in vitro and ex vivo experiments are essential for permitting experiments on animal models and for preclinical and clinical studies that precede the introduction of new bioactive molecules into pharmaceutical production. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The output brings new knowledge about the influence of the carotenoid astaxanthin and its fractions on cell metabolism, especially on their antioxidant mechanism of action. The doctoral students (Jurčacková Z., Ciglanová, D.), who worked on this research, significantly expanded their methodological and knowledge background during the implementation of the experiments. The knowledge gained will be further used for the education of students in the 3rd stage of university studies in the program: Microbiology and Immunology. |
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