KULKARNI, Amod - JOZEFIAKOVÁ, Jana - BHIDE, Katarína - MOCHNÁČOVÁ, Evelína - BHIDE, Mangesh Ramesh. Differential transcriptome response of blood brain barrier spheroids to neuroinvasive Neisseria and Borrelia. In: Frontiers in Cellular and Infection Microbiology. 2023. ISSN 2235-2988, Vol. 13, no. (2023), art. no. 1326578, p. [1-16] (2023: 4.600 - IF, 5.100 - IF 5y, 1.285 - SJR, Q1 - SJR Best Q, Q1

KULKARNI, Amod - JOZEFIAKOVÁ, Jana - BHIDE, Katarína - MOCHNÁČOVÁ, Evelína - BHIDE, Mangesh Ramesh. Differential transcriptome response of blood brain barrier spheroids to neuroinvasive Neisseria and Borrelia. In: Frontiers in Cellular and Infection Microbiology. 2023. ISSN 2235-2988, Vol. 13, no. (2023), art. no. 1326578, p. [1-16] (2023: 4.600 - IF, 5.100 - IF 5y, 1.285 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q)

Basic data
OCA1. Surname awarded to the assessed person	Bhide
OCA2. Name awarded to the assessed person	Mangesh Ramesh
OCA3. Degrees awarded to the assessed person	prof. MVSc., PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6102
OCA5. Area of assessment	habilitation and inauguration procedure – microbiology and immunology
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2023
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	1116774
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildC2VQT1&sid=82E8E2F14D9232B3C0ACA36D4D93&seo=CREPČ-detail-Článok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1326578/full
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	KULKARNI, Amod - JOZEFIAKOVÁ, Jana - BHIDE, Katarína - MOCHNÁČOVÁ, Evelína - BHIDE, Mangesh Ramesh. Differential transcriptome response of blood brain barrier spheroids to neuroinvasive Neisseria and Borrelia. In: Frontiers in Cellular and Infection Microbiology. 2023. ISSN 2235-2988, Vol. 13, no. (2023), art. no. 1326578, p. [1-16] (2023: 4.600 - IF, 5.100 - IF 5y, 1.285 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q)
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1326578/full
OCA14. Characteristics of the author's contribution	The author is the bearer of ideas and the main researcher of all research in the field
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.
Other data
OCA16. Annotation of the output in English	The self-assembling spheroids were used to perceive the BBB response to neuroinvasive pathogens - Neisseria and Borrelia. Compromised integrity of spheroids during Neisseria and Borrelia infection as opposed to its intactness and non-adherence of E. coli (non-neuroinvasive) denotes the pathogen dependent fate of BBB. Genes categorized into various biological functions indicated weakened barrier properties of BBB and heightened innate immune response. Inverse expression of 48% genes commonly identified during Neisseria and Borrelia infection exemplifies unique response of BBB to varying neuropathogens.
OCA17. List of maximum 5 most significant citations corresponding to the output	2026: [1] DELBAZ, Ali - ST JOHN, James A. Outer Membrane Vesicles as Systems-Level Drivers of Neuroinflammation, Metabolic Dysfunction, and Proteinopathy in Alzheimer\'s Disease. In CELLS, 2026, vol. 15, no. 8, pp. Dostupné na: https://doi.org/10.3390/cells15080690. 2026: [1] KOSTKA-WIRTZ, Kathrin - BIALAS, Nataniel - KOSTINA, Ivanna - EPPLE, Matthias. Permeability of the Blood-Brain Barrier (BBB) to Nanoparticles, Bacteria and Phages Studied in BBB Organoids Under Normoxic and Hypoxic Conditions In Vitro. In ORGANOIDS, 2026, vol. 5, no. 1, pp. Dostupné na: https://doi.org/10.3390/organoids5010001. 2025: [1] LIU, Huazhen - KONG, Xingxing - ZENG, Yuqin - CHEN, Jinyun - CHEN, Zhanpeng - LIU, Lanlan - MA, Quan - LIU, Xuhui - LU, Shuihua. From pain to meningitis: bacteria hijack nociceptors to promote meningitis. In FRONTIERS IN IMMUNOLOGY, 2025, vol. 15, no., pp. ISSN 1664-3224. Dostupné na: https://doi.org/10.3389/fimmu.2024.1515177. 2026: [1] MATEO, Ana M. - TRONCOSO-AFONSO, Lara - PERIN, Francesca - GARCIA-ASTRAIN, Clara. 3D (Bio)printed In Vitro Infection Models: Tools to Investigate Host-Pathogen Interactions and Accelerate the Discovery of Therapies. In ACS APPLIED POLYMER MATERIALS, 2026, vol. 8, no. 2, pp. 725-742. ISSN 2637-6105. Dostupné na: https://doi.org/10.1021/acsapm.5c04079. 2026: [1] NEUMANN, Natanel - JACOBSON, Moriah L. - BREHM, Michael A. - DANIELS, Keith A. - KADY, Jamie - O\'DONNEL, Carey L. - ROGOVSKA, Yuliya - WANG, Sichao - ROGOVSKYY, Artem S. Testing the capacity of humanized immune system mice to induce a protective antibody response against the Lyme disease pathogen. In MICROBIOLOGY SPECTRUM, 2026, vol. 14, no. 5, pp. Dostupné na: https://doi.org/10.1128/spectrum.03803-25.
OCA18. Characteristics of the output's impact on socio-economic practice	The output elucidates the biological processes taking place in brain endothelial cells after their induction by Streptococcus pneumoniae (SP) and its ligand laminin binding protein. Data from transcriptome analysis were processed using innovative bioinformatics tools. The elucidation of cell transcriptome forms the basis for understanding the process of SP translocation across the blood-brain barrier. These findings can be used in the development of ligand-blocking therapeutic agents.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output describes methods of culturing and inducing SP endothelial cells, as well as a method of isolating and processing RNA for RNA sequencing. Transcriptome analysis was performed using innovative bioinformatics tools. All modern procedures presented in this output can be applied in the educational process as a model for biological processing of samples, as well as the analysis of transcriptome data. .