| Basic data |
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| OCA1. Surname awarded to the assessed person | Bhide |
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| OCA2. Name awarded to the assessed person | Mangesh Ramesh |
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| OCA3. Degrees awarded to the assessed person | prof. MVSc., PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6102 |
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| OCA5. Area of assessment | habilitation and inauguration procedure – microbiology and immunology |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2021 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | ID = 254702 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioFormChildE14H0M&sid=1D6E60CFDFB4EEDFDBCCD68B45&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://www.scopus.com/record/display.uri?eid=2-s2.0-85104108329&origin=resultslist&sort=plf-f&src=s&st1=mochnacova&st2=Evel%c3%adna&nlo=1&nlr=20&nls=count-f&sid=ac5d890c4e82e68475cb324624ac36cf&sot=anl&sdt=aut&sl=40&s=AU-ID%28%22Mochn%c3%a1%c4%8dov%c3%a1%2c+Evel%c3%adna%22+57221393156%29&relpos=0&citeCnt=0&searchTerm= |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | JIMÉNEZ-MUNGUÍA, Irene - TOMEČKOVÁ, Zuzana - MOCHNÁČOVÁ, Evelína - BHIDE, Katarína - MAJEROVÁ, Petra - BHIDE, Mangesh Ramesh. Transcriptomic analysis of human brain microvascular endothelial cells exposed to laminin binding protein (adhesion lipoprotein) and Streptococcus pneumoniae. In: Scientific Reports. 2021. ISSN 2045-2322, Vol. 11, no. 1 (2021), art. no. 7970, p. [1-15]
(2021: 4.997 - IF, 242 - H-index, 5.516 - IF 5y, 1.005 - SJR, Q1 - SJR Best Q, Q2 - JCR Best Q) |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.nature.com/articles/s41598-021-87021-4 |
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| OCA14. Characteristics of the author's contribution | The author is the bearer of ideas and the main researcher of all research in the field. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | |
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| Other data |
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| OCA16. Annotation of the output in English | Streptococcus pneumoniae invades the CNS and triggers a strong cellular response. To date, signaling events that occur in the human brain microvascular endothelial cells (hBMECs), in response to pneumococci or its surface adhesins are not mapped comprehensively. We evaluated the response of hBMECs to the adhesion lipoprotein (a laminin binding protein—Lbp) or live pneumococci. Lbp is a surface adhesin recently identified as a potential ligand, which binds to the hBMECs. Transcriptomic analysis was performed by RNA-seq of three independent biological replicates and validated with qRT-PCR using 11 genes. In total 350 differentially expressed genes (DEGs) were identified after infection with S. pneumoniae, whereas 443 DEGs when challenged with Lbp. Total 231 DEGs were common in both treatments. Integrative functional analysis revealed participation of DEGs in cytokine, chemokine, TNF signaling pathways and phagosome formation. Moreover, Lbp induced cell senescence and breakdown, and remodeling of ECM. This is the first report which maps complete picture of cell signaling events in the hBMECs triggered against S. pneumoniae and Lbp. The data obtained here could contribute in a better understanding of the invasion of pneumococci across BBB and underscores role of Lbp adhesin in evoking the gene expression in neurovascular unit. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | 2025: [1] MAUSER, Henry D. - KEYZER, Taryn E. - SURMA, Jessica M. - ALEXANDER, Natalie G. - CUTTS, William D. - HATHCOCK, Sarah F. - LACKEY, Kimberly H. - LAM, Daryl W. - THORNTON, Justin A. - VOLLMUTH, Nadine - KIM, Brandon J. Stem cell-derived brain-like endothelial cells to interrogate Streptococcus pneumoniae interaction with brain endothelium. In VIRULENCE, 2025, vol. 16, no. 1, pp. ISSN 2150-5594. Dostupné na: https://doi.org/10.1080/21505594.2025.2564281.
2024: [1] SMITH, Frances M. - KOSMAN, Daniel J. Loss of filamentous actin, tight junction protein expression, and paracellular barrier integrity in frataxin-deficient human brain microvascular endothelial cells—implications for blood-brain barrier physiology in Friedreich’s ataxia. In Frontiers in Molecular Biosciences, 2024-01-11, 10, pp. Dostupné na: https://doi.org/10.3389/fmolb.2023.1299201.
2024: [1] ZHAO, Meng - WANG, Yongchun - SHEN, Yanxin - WEI, Chunxiao - ZHANG, Guimei - SUN, Li. A review of the roles of pathogens in Alzheimer’s disease. In Frontiers in Neuroscience, 2024-01-01, 18, pp. ISSN 16624548. Dostupné na: https://doi.org/10.3389/fnins.2024.1439055.
2024: [1] ZHAO, Meng - WANG, Yongchun - SHEN, Yanxin - WEI, Chunxiao - ZHANG, Guimei - SUN, Li. A review of the roles of pathogens in Alzheimer’s disease. In Frontiers in Neuroscience, 2024-01-01, 18, pp. ISSN 16624548. Dostupné na: https://doi.org/10.3389/fnins.2024.1439055.
2023: [1] Payen, S. - Rrodriguez, J.A. - Segura, M. - Gottschalk, M. Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses. In VETERINARY RESEARCH. ISSN 0928-4249, 2023, vol. 54, no. 1, art. no. 1, p. [1-13]. [WoS] |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The output elucidates the biological processes taking place in brain endothelial cells after their induction by Streptococcus pneumoniae (SP) and its ligand laminin binding protein. Data from transcriptome analysis were processed using innovative bioinformatics tools. The elucidation of cell transcriptome forms the basis for understanding the process of SP translocation across the blood-brain barrier. These findings can be used in the development of ligand-blocking therapeutic agents. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The output describes methods of culturing and inducing SP endothelial cells, as well as a method of isolating and processing RNA for RNA sequencing. Transcriptome analysis was performed using innovative bioinformatics tools. All modern procedures presented in this output can be applied in the educational process as a model for biological processing of samples, as well as the analysis of transcriptome data. |
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