| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | MOCHNÁČOVÁ, Evelína - BHIDE, Katarína - KUCKOVÁ, Katarína - JOZEFIAKOVÁ, Jana - MAĽARIK, Tomáš - BHIDE, Mangesh Ramesh. Antimicrobial cyclic peptides effectively inhibit multiple forms of Borrelia and cross the blood-brain barrier model. In: Scientific Reports. 2025. ISSN 2045-2322, Vol. 15, no. 1 (2025), art. no. 6147, p. [1-16]
(2025: 4.900 - IF, 382 - H-index, 4.800 - IF 5y, 0.893 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q) |
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| OCA16. Annotation of the output in English | Infection caused by neuroinvasive Borrelia often manifests long-term CNS disorders and is difficult to treat as most antibiotics fail to attain an effective concentration within the brain or cannot kill the persister forms of Borrelia. Thus, this study focused on developing antimicrobial cyclic peptides (AMPs) from a combinatorial phage display library that target phosphatidylcholine of the borrelial cell membrane. Isolated cyclic peptides with anti-Borrelia properties were then fused with the CNS homing peptide developed in this study (designated as O-BBB) to facilitate AMP transport across the blood-brain barrier. Among all O-BBB fused AMPs, Bor-18 had half maximal effective concentration (EC50) 0.83 µM when tested against spirochetal Borrelia. Bor-16, Bor-18, and Bor-26 inhibited the cystic form with EC50 0.83 µM, while Bor-11 had EC50 0.41 µM. Within an hour, all four peptides caused a permeability breach in the borrelial cell membrane, causing depolarization of the membrane. Bor peptides did not inhibit eukaryotic cell metabolism or proliferation, nor did they cause erythrocyte lysis. Peptides were stable in serum, could cross the BBB in-vitro, and remained effective against Borrelia. Cyclic AMPs fused with a CNS homing moiety, the Bor peptides, deserve further investigation for their potential use in neuroborreliosis therapy. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | 2026: [1] DING, Wenxiu - WANG, Xiao - TIAN, Jingwen - YANG, Guangpu - MA, Xuanxuan - LI, Xiuxiu - XU, Jing. The Sources, Synthesis, and Applications of Cyclopeptides. In MACROMOLECULAR BIOSCIENCE, 2026, vol. 26, no. 2, pp. ISSN 1616-5187. Dostupné na: https://doi.org/10.1002/mabi.202500484.
2025: [1] HALAWA, Mohamed - GALLO, Alicia L. - CARABETTA, Valerie J. Structural and Functional Insights into Viral and Fungal Proteins Involved in Chronic Inflammation and Their Biologic Treatments. In PHARMACEUTICS, 2025, vol. 17, no. 11, pp. Dostupné na: https://doi.org/10.3390/pharmaceutics17111466.
2025: [1] KAKISH, Diala Ra Ed Kamal - ALSAMHORI, Jehad Feras - AL-SAWALHA, Mohammed - SHEHADA, Wafaa - AL SALKHADI, Asham - PRASHASTI, D. - ALMALITI, Ibrahim - AL-ZURIQAT, Mohammad - AKASHEH, Qais - NASHWAN, Abdulqadir J. Innovative screening methods to reduce the misdiagnosis of Lyme disease: Public health and global implications. In Microbe Netherlands, 2025-06-01, 7, pp. Dostupné na: https://doi.org/10.1016/j.microb.2025.100366.
2025: [1] LI, Wenhao - ZHU, Geyi - ZUBAIR, Muhammad - GUO, Cui - ZHANG, Lin - LU, Peicheng - YAN, Yongmin - CHU, Ying - ZHANG, Haiyan - HAN, Guomin. Deep Learning-Driven Discovery of Novel Antimicrobial Peptides from Large-Scale Protist Genomes and Experimental Characterization. In JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2025, vol. 65, no. 19, pp. 9962-9973. ISSN 1549-9596. Dostupné na: https://doi.org/10.1021/acs.jcim.5c01196.
2025: [1] VINUKONDA, Anjaneyulu - RAPOLU, Kishore - JADI, Rajendra Kumar - DEVADASU, Venkat Ratnam. Complex Peptide Injectables: Development and Challenges. In International Journal of Peptide Research and Therapeutics, 2025-05-01, 31, 3, pp. ISSN 15733149. Dostupné na: https://doi.org/10.1007/s10989-025-10710-5. |
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