\"LOZIAKOVÁ PEŇAZZIOVÁ, Katarína - SCHUSTEROVÁ, Petra - PIVKA, Soňa - PAČANSKÁ, Zuzana - SZEMES, Tomáš - KLEMPA, Boris - CSANK, Tomáš. Detection, genetic characterization and reassortment analysis of Tribeč virus. In: Archives of virology. 2026. ISSN 0304-8608, Vol. 171, no. 4 (2026), art. no. 107, p. [1-15] (2024: 2.500 - IF, 135 - H-index, 2.200 - IF 5y, 0.671 - SJR, Q2 - SJR Best Q, Q3 - JCR Best Q)\"

Basic data
OCA1. Surname awarded to the assessed personCsank
OCA2. Name awarded to the assessed personTomáš
OCA3. Degrees awarded to the assessed personAssoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staffhttps://www.portalvs.sk/regzam/detail/6133
OCA5. Area of assessmenthabilitation and inauguration procedure – microbiology and immunology
OCA6. Category of the research/artistic/other outputscientific output
OCA7. Year of publication of the research/artistic/other output2026
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)1541406
OCA9. Hyperlink to the record in CRPA or CRAAhttps://app.crepc.sk/?fn=detailBiblioForm&sid=F065241B7212E93A27176D33722A
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputshttps://www.scopus.com/pages/publications/105031625825?origin=resultslist
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs\"LOZIAKOVÁ PEŇAZZIOVÁ, Katarína - SCHUSTEROVÁ, Petra - PIVKA, Soňa - PAČANSKÁ, Zuzana - SZEMES, Tomáš - KLEMPA, Boris - CSANK, Tomáš. Detection, genetic characterization and reassortment analysis of Tribeč virus. In: Archives of virology. 2026. ISSN 0304-8608, Vol. 171, no. 4 (2026), art. no. 107, p. [1-15] (2024: 2.500 - IF, 135 - H-index, 2.200 - IF 5y, 0.671 - SJR, Q2 - SJR Best Q, Q3 - JCR Best Q)\"
OCA12. Type of the output (if the output is not registered in CRPA or CRAA) article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)https://link.springer.com/article/10.1007/s00705-026-06597-2
OCA14. Characteristics of the author's contributionThe author is the bearer of ideas and the main researcher of all research in the field
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.
Other data
OCA16. Annotation of the output in EnglishThis study reports the detection and genetic characterization of Tribeč virus (TRBV), a tick-borne orbivirus of zoonotic relevance. The virus was identified in Ixodes ricinus ticks collected from a goat using a newly developed RT-PCR assay. Goats grazing in the study area showed seroconversion, with low titre neutralization antibody response. The new isolate, designated 16.C/2016/Dubrava/SVK, was successfully cultured in Vero E6 cells and detected by immunofluorescence. Replication efficiency varied across rodent, human, and bovine cell lines. Whole-genome sequencing revealed that most genome segments shared high sequence identity with other TRBV strains and with Muko virus (MUKV) strains from Japan. In contrast, phylogenetic analysis based on the VP4 outer capsid protein indicated that the TRBV clade is more closely related to Kemerovo viruses from Russia. Segment 10 showed the highest variability, where strains from Slovakia and Romania formed sister clades with MUKV, and the Ukrainian strains with KEMV. Indication of a reassortment was observed in segment 4, encoding the NS1 tubule-forming protein, which appears to originate from the prototype TRBV isolated in Slovakia during the 1960s. Collectively, these findings highlight the genetic variability of TRBV and support the need for further studies on the ecology, evolution, and diversity of tick-borne orbiviruses.
OCA17. List of maximum 5 most significant citations corresponding to the outputnie sú
OCA18. Characteristics of the output's impact on socio-economic practiceResearch into the Tribeč virus (TRBV) brings a newly developed RT-PCR test into social -economic practice a newly developed RT-PCR test ready for routine monitoring in laboratories, whilst also highlighting the zoonotic risk, as the virus’s ability to replicate in human cells and its transmission by the common tick mean it must be considered in cases of unexplained illness following a tick bite. For agriculture and veterinary practice, the results have a direct impact on the management of livestock rearing and grazing (goats and cattle), whilst the genetic variability and reassortment of the virus that have been identified are key to early prevention and the avoidance of the emergence of more pathogenic strains.
OCA19. Characteristics of the output and related activities' impact on the educational processResearch into the Tribeč virus enriches education in the fields of medicine, veterinary science and biology by providing a real-world example of the One Health concept, whilst updating the curriculum to include lesser-known arboviruses. The newly developed RT-PCR protocol, the Vero E6 cell culture methodology and immunofluorescence serve as direct guides for laboratory exercises, whilst data from whole-genome sequencing provide practical material for teaching bioinformatics and phylogenetic analyses of viral reassortment. The knowledge gained also generates attractive topics for final-year university theses and forms the basis for the continuing professional development of doctors and vets in the field of differential diagnosis of tick-borne diseases.

Last updated: 28.10.2026

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