ADC Antimicrobial resistance and species identification of staphylococci isolated from the meat of wild rabbits (Oryctolagus cuniculus) in Slovakia / Pipová Monika, Jevinová Pavlína, Kmeť Vladimír, Regecová Ivana, Marušková Katarína. In: European Journal of Wildlife Research. - ISSN 1612-4642.

ADC Antimicrobial resistance and species identification of staphylococci isolated from the meat of wild rabbits (Oryctolagus cuniculus) in Slovakia / Pipová Monika, Jevinová Pavlína, Kmeť Vladimír, Regecová Ivana, Marušková Katarína. In: European Journal of Wildlife Research. - ISSN 1612-4642. - Vol. 58, no. 1 (2012), p. 157-165

Basic data
OCA1. Surname awarded to the assessed person	Pipová
OCA2. Name awarded to the assessed person	Monika
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, CSc.
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6030
OCA5. Area of assessment	study programme food hygiene · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2012
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	UVL.Košice.0014878
OCA9. Hyperlink to the record in CRPA or CRAA	http://www.crepc.sk/portal?fn=*recview&uid=739900&pageId=resultform&full=0
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000301781700017
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Antimicrobial resistance and species identification of staphylococci isolated from the meat of wild rabbits (Oryctolagus cuniculus) in Slovakia / Pipová Monika, Jevinová Pavlína, Kmeť Vladimír, Regecová Ivana, Marušková Katarína. In: European Journal of Wildlife Research. - ISSN 1612-4642. - Vol. 58, no. 1 (2012), p. 157-165
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://link.springer.com/article/10.1007/s10344-011-0558-2
OCA14. Characteristics of the author's contribution	The author participated in the processing of samples for microbiological testing, isolation and identification of staphylococcal species, as well as in testing their susceptibility to selected antibiotics using the disk diffusion method according to Kirby-Bauer. She also wrote the manuscript, made all corrections in accordance with reviewers\' comments as well as the final revision before publication.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	A total of 113 staphylococci isolates were obtained from the thigh muscles of ten hunted wild rabbits and twenty farmed rabbits (Oryctolagus cuniculus) in the Slovak Republic.Most isolates (98.2%) were coagulase-negative and six of them (5.4%) produced thermostable DNase. Till now, no data are available on the species iand antimicrobial resistance of coagulase-negative staphylococci present in rabbit meat. Therefore, species identification was performed using the MALDI BioTyper sytem. The following species of staphylococci were identified: Staphylococcus aureus (1.8%), Staphylococcus warneri (45.1%), Staphylococcus epidermidis (21.2%), Staphylococcus pasteuri (13.3%), Staphylococcus xylosus (8.0%), Staphylococcus capitis (7.1%), Staphylococcus haemolyticus (1.8%) and Staphylococcus cohnii ssp. cohnii (1.8%). The agar dilution method was used to test resistance of isolates to eight antibiotics. Only one strain was susceptible to all antibiotics tested, all the other strains were resistant to at least one antibiotic, where resistance to ampicillin (78.8%), was followed by resistance to erythromycin (58.4%), penicillin (51.3%) and oxacillin (46.0%). In contrary, most strains were susceptible to cefoxitin (92.0%) and gentamicin (98.2%). As seen from the results of this study, coagulase-negative staphylococci, although being present in the meat of wild rabbits in low concentrations, can serve as important donors of antibiotic resistance genes.
Other data
OCA16. Annotation of the output in English	In 2009, a total of 113 strains of staphylococci were isolated from the thigh muscles of ten hunted and 20 farmed wild rabbits (Oryctolagus cuniculus) in the Slovak Republic. In each isolate, resistance to eight antibiotics by means of agar dilution test was tested. Based on these results, 110 isolates were found to be resistant to at least one antibiotic. Resistance to ampicillin (78.8%), erythromycin (58.4%), penicillin (51.3%) and oxacillin (46.0%) was found most frequently. Twenty-six isolates (23.0%) were resistant to novobiocin. On the other hand, resistance to cefoxitin (8.0%) and gentamicin (1.8%) were quite rare. Fifteen percent of isolates were resistant to one antibiotic, simultaneous resistance to two, three, four and five antibiotics was confirmed in 22.1%, 23.9%, 21.2% and 13.3% of isolates, respectively. Except for two coagulase-positive Staphylococcus aureus isolates (1.8%), seven species of coagulase-negative staphylococci were identified using the MALDI-TOF mass spectrometry as follows: S. warneri (45.1%), S. epidermidis (21.2%), S. pasteuri (13.3%), S. xylosus (8.0%), S. capitis (7.1%), S. haemolyticus (1.8%) and S. cohnii ssp. cohnii (1.8%).
OCA17. List of maximum 5 most significant citations corresponding to the output	BEZJIAN, Marisa - KOLLIAS, George V. American Kestrel (Falco spaverius) Fledgling With Severe Bilateral Periorbital Swelling and Infection With Mycoplasma buteonis, Avibacterium (Pasteurella) gallinarum, and Staphylococcus pasteuri. In JOURNAL OF AVIAN MEDICINE AND SURGERY. ISSN 1082-6742, 2014, vol. 28, no. 2, pp. 127. EID, EID, Samah - SAMIR, Abdel Hafeez. Antimicrobial resistance attributes of staphylococci isolated from rabbits. In BIOSCIENCE RESEARCH. ISSN 1811-9506, 2018, vol. 15, no. 2, pp. 862-872. SILVA, Vanessa - PEREIRA, Jose Eduardo - MALTEZ, Luis - FERREIRA, Eugenia - MANAGEIRO, Vera - CANICA, Manuela - CAPELO, Jose L. - IGREJAS, Gilberto - POETA, Patricia. Diversity of methicillin-resistant staphylococci among wild Lepus granatensis: first detection of mecA-MRSA in hares. In FEMS MICROBIOLOGY ECOLOGY. ISSN 0168-6496, 2020, vol. 96, no. 1, pp. [WoS] SOUSA, Margarida - SILVA, Vanessa - SILVA, Adriana - SILVA, Nuno - RIBEIRO, Jessica - TERESA TEJEDOR-JUNCO, Maria - CAPITA, Rosa - SAFIA CHENOUF, Nadia - ALONSO-CALLEJA, Carlos - RODRIGUES, Tiago M. - LEITAO, Manuel - GONCALVES, David - CANICA, Manuela - TORRES, Carmen - IGREJAS, Gilberto - POETA, Patricia. Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? In JOURNAL OF FOOD PROTECTION. ISSN 0362-028X, 2020, vol. 83, no. 7, pp. 1110-1114. MATSUNAGA, Nonoka - SUZUKI, Mariko - ANDOH, Masako - IJIRI, Moe - ISHIKAWA, Kemi - OBI, Takeshi - CHUMA, Takehisa - FUJIMOTO, Yoshikazu. Analysis of fecal samples from Amami rabbits (Pentalagus furnessi) indicates low levels of antimicrobial resistance in Escherichia coli. In EUROPEAN JOURNAL OF WILDLIFE RESEARCH. ISSN 1612-4642, 2020, vol. 66, no. 5, pp.
OCA18. Characteristics of the output's impact on socio-economic practice	The emergence of antimicrobial resistance among food bacteria is an increasing problem worldwide and the food chain is considered an important way of transmitting resistant bacteria between animal and human populations. Staphylococci are widespread in nature and are therefore regularly isolated from humans, domestic and wild animals, as well as from various food products. Currently, medical attention is paid mainly to coagulase-negative staphylococci as the major activators of nosocomial infections leading to serious therapeutic failures. As reported in this study, coagulase-negative staphylococci, although present in the meat of wild rabbits in low concentrations in the meat of wild rabbits, may serve as important donors of resistance genes. Because wild animals move across the country, they can easily come into contact with industrial, agricultural and municipal wastes as well as with food-producing animals or pets. Thus, antibiotic resistance can be transferred to their normal microbial flora even if they have never been treated with antimicrobials. To ensure food safety, permanent susceptibility testing of food bacteria to antimicrobials is of great importance as it provides actual and reliable information on the level of resistance in microbial food populations.
OCA19. Characteristics of the output and related activities' impact on the educational process	Susceptibity testing of food microorganisms to antibiotics used in human and veterinary medicine is included into into teaching programme of the compulsory subject \"Food Microbiology and Foodborne Diseases\" in the postgraduate study.