| Basic data |
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| OCA1. Surname awarded to the assessed person | Kožárová |
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| OCA2. Name awarded to the assessed person | Ivona |
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| OCA3. Degrees awarded to the assessed person | Assoc. prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6056 |
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| OCA5. Area of assessment | study programme food hygiene · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2020 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 185193 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=5EBE627ECA7CA644E3D7DB4608 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://www.webofscience.com/wos/woscc/full-record/WOS:000533028100001 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | ADC KOŽÁROVÁ, Ivona - JUŠČÁKOVÁ, Daniela - ŠIMKOVÁ, Jana - MILKOVIČOVÁ, Mária - KOŽÁR, Martin. Effective screening of antibiotic and coccidiostat residues in food of animal origin by reliable broad-spectrum residue screening tests. In Italian Journal of Animal Science. ISSN 1594-4077, 2020, vol. 19, no. 1, p. 487-501. (2019: 1.805 - IF, 1.697 - IF 5y, 32 - H-index, 0.534 - SJR, Q2 - SJR Best Q, Q1 - JCR Best Q). |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.tandfonline.com/doi/full/10.1080/1828051X.2020.1761270 |
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| OCA14. Characteristics of the author's contribution | The author is the bearer of the main ideas and the first author of the scientific output. She is also the bearer of funds for the project from which the scientific output was realized. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | Summarising the results of all positive results of all tube tests and the STAR method, 62 samples were positive (13 of which were dubious) by at least one tube test and 65 samples were positive as determined by the STAR method. The additional post-screening analysis of beta-lactams or sulphonamides in the screen-positive samples using penicillinase and PABA revealed the following: 1 porcine muscle sample and 20 milk samples were positive for beta-lactam antibiotics; and all chicken livers (10), 2 kidneys, 2 hearts, 3 fats/skins, 2 spleens and 2 gizzards and 5 eggs were positive for sulphonamides. Based on the screening and post-screening results obtained, we can conclude that positive chicken livers, heart, fats/skins, spleens and gizzards are suspected for the presence of sulphonamides, chicken kidneys for the presence of sulphonamides and aminoglycosides, chicken muscle for the presence of aminoglycosides, porcine muscle for the presence of beta-lactams, egg samples for the presence of sulphonamides and macrolides, and milk samples for the presence of beta-lactams and tetracyclines. |
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| Other data |
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| OCA16. Annotation of the output in English | Residue control is very important to ensure food safety. Microbial inhibition tests are methods of choice for in the initial screening of antibiotic residues in food of animal origin. In spite of the fact that microbial inhibition tests do not provide the data on sensitivity to coccidiostats, the antibacterial activity of salinomycin allows us to confirm our previous findings and contribute additional evidence to the fact that the microbial inhibition tests which use the test organism Bacillus stearothermophilus var. calidolactis as their test organism are suitable for the effective detection of salinomycin residues in food of animal origin. The spectrum of antimicrobials detectable by the screening methods described is not exhaustive and there is still scope for its further expansion and improvement. The outcomes of our study may also be promising with respect to the unexpected finding that PABA counteracted the inhibition of salinomycin on the test organism Bacillus stearothermophilus var. calidolactis. This means that in addition to beta-lactams and sulphonamides, the presence of coccidiostats should also be taken into account. The two-tier testing system based on the microbial screening and more specific post-screening is proven to be an effective tool in controlling residues of antimicrobial substances in food of animal origin and, equally, is an effective tool in the initial screening of coccidiostat residues in poultry meat and eggs. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | MA, Chi - WEI, Jingjing - JIANG, Kainian - YANG, Zhongzhu - YANG, Xu - YANG, Kaihua - ZHANG, Yi - ZHANG, Chang. Self-assembled micro-flowers of ultrathin Au/BiOCOOH nanosheets photocatalytic degradation of tetracycline hydrochloride and reduction of CO
In CHEMOSPHERE. ISSN 0045-6535, 2021, vol. 283, no., pp., Registrované v: WoS 2. XU, Yifei - BONIZZONI, Marco. Beta-lactam antibiotic discrimination using a macromolecular sensor in water at neutral ph. In Sensors. ISSN 14248220, 2021-10-01, 21, 19, pp., Registrované v: Scopus
LI, Tao - ZHANG, Shuya - ZHANG, Jiqiao - SONG, Yiping - BAO, Xiuyu - XU, Fengwen - ZHANG, Jianqin. Analysis of Serum Biochemical Indexes, Egg Quality, and Liver Transcriptome in Laying Hens Fed Diets Supplemented with Gynostemma pentaphyllum Powder. In GENES, 2021, vol. 12, no. 12, pp. Dostupné na: https://doi.org/10.3390/genes12121942., Registrované v: WoS
GARMASHOV, Sergey Yurievich - CHAPLYGINA, Olga Sergeevna. Using the HPLC-MS/MS method to determine the antibiotic amphenicols group in livestock products. In Food Technology, 2021, vol. 10, no. 2, p. 113-117. DOI: 10.46548/21vek-2021-1054-0021
. PROSEKOV, Alexandr – PODLEGAEVA, Tatiana – CHAPLYGINA, Olga – KOZLOVA, Oksana – TULAEVA, Anna. Control of animal products contamination with nitroimidazole group antibiotics. In E3S Web of Conferences, vol. 222, p. 06019. EDP Sciences. https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/82 /e3sconf _ daic 2020_06019.pdf, Registrované v: Scopus |
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| OCA18. Characteristics of the output's impact on socio-economic practice | Our results confirmed the antibacterial activity of coccidiostat salinomycin on Gram-positive bacteria used as the test organisms of the microbial inhibition tests, which are used as the main method for the screening of antibiotic residues in products of animal origin. Interestingly, the results also revealed the unexpected finding that PABA antagonizes the antibacterial action of salinomycin on test organism Bacillus stearothermophilus var. calidolactis. This means that in addition to beta-lactams and sulphonamides, the presence of coccidiostats should also be taken into account. As, until now, there is no existing literature on this fact and no other studies are published for comparison of our results in this subject area, further experimental studies are needed to build on this finding, to understand the principle of the specific interaction between salinomycin and PABA and to verify the effect of PABA on the antibacterial activity of the other coccidiostats authorised for food-producing animals. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The outputs of the scientific article will be used in the implementation of scientific research activities of postgraduate education of doctoral students and as educational material presenting the legislation on official control of residues in products of animal origin. |
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