ADC Assessment of two different methods for sampling and detection of Dichelobacter nodosus and Fusobacterium necrophorum in dairy cows in Eastern Slovakia. Segurado Benito Pilipčincová, IvanaKirály, JánDolník, MichalOsová, AdrianaMudroň, Pavol. In Journal of Applied Animal Research 2018, Roč. 46, č. 1, s. 1452-1456

Basic data
OCA1. Surname awarded to the assessed person	Mudroň
OCA2. Name awarded to the assessed person	Pavol
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD, Dip. ECBHM
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6004
OCA5. Area of assessment	study programme internal diseases of animals · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2018
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	97532
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=057E6E723D4374EE26A4A0B6
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/record/display.uri?eid=2-s2.0-85063991901&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=64150edc57660b307f2b2f1b9b34e23b&sot=b&sdt=b&sl=23&s=AUTHOR-NAME+%28Mudron+P.%29&relpos=10&citeCnt=2&searchTerm=
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Assessment of two different methods for sampling and detection of Dichelobacter nodosus and Fusobacterium necrophorum in dairy cows in Eastern Slovakia. Segurado Benito Pilipčincová, IvanaKirály, JánDolník, MichalOsová, AdrianaMudroň, Pavol. In Journal of Applied Animal Research 2018, Roč. 46, č. 1, s. 1452-1456
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.tandfonline.com/doi/full/10.1080/09712119.2018.1532903
OCA14. Characteristics of the author's contribution	The author is the bearer of main ideas and co-author of the output. At the same time, he is also the holder of financial resources for projects from which the output was implemented.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The aim of our study was to find the most appropriate method of sampling bovine limbs, as well as to assess simple and efficient sample processing, including DNA extraction, for reliable diagnosis of Dichelobacter nodosus and Fusobacterium necrophorum bacteria. Eleven clinically healthy cows were enrolled in the study and sampled using swabs (2 types: superficial swab and deep swab). Two isolation methods were used for DNA extraction: 1. freezing and boiling of samples, 2. commercial kit (Roche). PCR analysis of the samples showed no variation in the detection ratio of D. nodosus and F. necrophorum with the different swabbing methods. The highest detection sensitivity for both bacteria was achieved by culturing the samples in AB followed by DNA extraction by freezing and boiling. Culturing in anaerobic broth resulted in detection of D. nodosus and F. necrophorum in more than 95% and 27%, respectively. In conclusion, a simple \"surface\" swab is sufficient for the detection of the pathogens studied, and freezing and boiling of the sample proved to be the most suitable method of DNA extraction.
Other data
OCA16. Annotation of the output in English	The aim of our study was to find the most appropriate way of sample collection from cattle feet as well as to assess simple and effective sample processing, including DNA extraction for reliable diagnosis of bacteria Dichelobacter nodosus and Fusobacterium necrophorum. 11 clinically healthy cows were included in the study, from which swabbing samples (2 types: surface swab and deep swab) were taken. Two isolation methods were used for DNA extraction: 1. freezing and boiling the samples, 2. commercial kit (Roche). PCR analysis of the samples has not shown any variations in the detection ratio of D. nodosus and F. necrophorum at different swabbing methods. The highest sensitivity of the detection of both bacteria was reached with a cultivation of samples in AB with subsequent extraction of DNA with freezing and boiling. The cultivation in anaerobic broth resulted in the detection rate of D. nodosus and F. necrophorum in over 95% and 27%, respectively. To conclude, the simple ‘surface’ swab is sufficient to detect studied pathogens, the most appropriate method of DNA extraction has proven to be freezing and boiling of the sample.
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	The output of the study is focused on cattle breeders and veterinarians, who can use them in the diagnosis of hoof diseases, especially in the differentiation of infectious and non-infectious forms.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output of the study is used in the teaching of Diseases of ruminants and Herd Health Management.