| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | ADC Characterization of two novel lactic acid bacteria isolated from the intestine of rainbow trout (Oncorhynchus mykiss, Walbaum) in Slovakia / Adriana Fečkaninová, Jana Koščová, Dagmar Mudroňová, Petra Schusterová, Ivana Cingeľová Maruščáková, Peter Popelka. In Aquaculture. - ISSN 0044-8486. - Vol. 506, no.(2019), p. 294-301. (2019). [FEČKANINOVÁ, Adriana (51%) - KOŠČOVÁ, Jana (10%) - MUDROŇOVÁ, Dagmar (10%) - SCHUSTEROVÁ, Petra (10%) - CINGEĽOVÁ MARUŠČÁKOVÁ, Ivana (9%) - POPELKA, Peter (10%)] |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The results indicate that all tested isolates R1–R6 would have good growth properties in rainbow trout aquaculture. Results from several existing probiotic clinical trials are both in- conclusive and conflicting. Several authors have described positive preventive or therapeutic effects of probiotics in various diseases of salmonids. However, other reports have not detected any, or significant, effects of probiotic microorganisms on the health status of aquatic animals. The scientific studies to date have shown that the use of probiotics are most effective during development of microbiota in the early life stages of fish, or when the stability of microbiota is disturbed. The conflicting results between probiotic studies could be due to many factors, such as selection of probiotic candidates, survival and stability of probiotics, species specificity of the strain in relation to the host, dosage, frequency and mode of administration of probiotics, health and nutritional status of fish, fish species and age, interactions with drugs or microbiota, stress, and general organization of experiments. |
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| OCA16. Annotation of the output in English | An effective probiotic must comply with criteria, which determines its effect. The aim of this work was to isolate lactic acid bacteria (LAB) from the intestinal content of rainbow trout (Oncorhynchus mykiss), subsequently potentially used as probiotics in order to improve health status of fish during fish farming. Selection criteria were used to obtain suitable probiotic candidates for aquaculture of salmonid. A total of 6 lactic acid bacteria isolated from intestines of rainbow trout belonging to the genus Lactobacillus were included in this study, and encompassed the following species: L. plantarum (n = 3), L. fermentum (n = 2), and L. brevis (n = 1). Antimicrobial susceptibility test has been performed on the basis of Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance by the EFSA. Sensitivity or intrinsic resistance of the isolated LAB to a recommended set of antibiotics make them safe for use as probiotics in aquaculture. All six autochthonous isolates showed the antagonistic activity against both salmonid pathogens Aeromonas salmonicida subsp. salmonicida CCM 1307 and Yersinia ruckeri CCM 6093. Determined in vitro survival conditions in the gastrointestinal tract of rainbow trout, and taking into account the previous results, two isolates - Lactobacillus plantarum (R2) and Lactobacillus fermentum (R3), which showed the highest level of tolerance to different pH values, bile, temperature, and the best growth properties, were selected as candidates for probiotics. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | Guangxin, G., Li, K., Zhu, Q., Zhao, C., Li, C., He, Z., Hu, S., Ren, Y. Improvements of immune genes and intestinal microbiota composition of turbot (Scophthalmus maximus) with dietary oregano oil and probiotics (2022) Aquaculture, 547, art. no. 737442.
Meidong, R., Buatong, A., Nakao, M., Sakai, K., Tongpim, S. Mixed culture of Bacillus aerius B81e and Lactiplantibacillus paraplantarum L34b-2 derived from in vivo screening using hybrid catfish exhibits high probiotic effects on Pangasius bocourti (2021) Journal of Bioscience and Bioengineering, 132 (4), pp. 423-428.
Meidong, R., Nakao, M., Sakai, K., Tongpim, S. Lactobacillus paraplantarum L34b-2 derived from fermented food improves the growth, disease resistance and innate immunity in Pangasius bocourti (2021) Aquaculture, 531, art. no. 735878.
Guo, G., Li, C., Xia, B., Jiang, S., Zhou, S., Men, X., Ren, Y. The efficacy of lactic acid bacteria usage in turbot Scophthalmus maximus on intestinal microbiota and expression of the immune related genes (2020) Fish and Shellfish Immunology, 100, pp. 90-97.
Liu, X., Shi, H., He, Q., Lin, F., Wang, Q., Xiao, S., Dai, Y., Zhang, Y., Yang, H., Zhao, H. Effect of starvation and refeeding on growth, gut microbiota and non-specific immunity in hybrid grouper (Epinephelus fuscoguttatus♀×E. lanceolatus♂) (2020) Fish and Shellfish Immunology, 97, pp. 182-193. |
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