| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | ADC Comparison of methods for measuring protein concentration in venom samples / Aleksandra Bocian, Sonja Slawek, Marcin Jaromin, Konrad Kamil Hus, Justyna Buczkowicz, Dawid Lysiak, Vladimír Petrilla, Monika Petrillová, Jaroslav Legáth. In Animals. - ISSN 2076-2615. - Vol. 10, no. 3 (2020), art. no. 448, p. [1-9]. (2019: 2.323 - IF, 28 - H-index, 0.601 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q). |
|---|
| OCA16. Annotation of the output in English | Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms. |
|---|
| OCA17. List of maximum 5 most significant citations corresponding to the output | ElSaboni, Y., Hunt, J.A., Stanley, J., Moffatt, C., Wei, Y.Development of a textile based protein sensor for monitoring the healing progress of a wound(2022) Scientific Reports, 12 (1), art. no. 7972
Okumu, M.O., Mbaria, J.M., Gikunju, J.K., Mbuthia, P.G., Madadi, V.O., Ochola, F.O., Maloba, K.N., Nderitu, J.G.Preclinical efficacy testing of three antivenoms against Naja ashei venom-induced lethality(2022) Toxicon: X, 14, art. no. 100124
Marrugo-Padilla, A., Méndez-Cuadro, D., Rodríguez-Cavallo, E.Tetracycline residues induce carbonylation of milk proteins and alter their solubility and digestibility(2022) International Dairy Journal, 125, art. no. 105226
Lemauf, S., Cazes, D., Poirié, M., Gatti, J.-L.Amount of venom that Leptopilina species inject into Drosophila melanogaster larvae in relation to parasitic success(2021) Journal of Insect Physiology, 135, art. no. 104320
Hus, K.K., Marczak, Ł., Petrilla, V., Petrillová, M., Legáth, J., Bocian, A.Different research approaches in unraveling the venom proteome of naja ashei(2020) Biomolecules, 10 (9), art. no. 1282, pp. 1-12 |
|---|
