ADC Effect of autochthonous lactobacilli on immunologically important molecules of rainbow trout after bacterial infection studied on intestinal primoculture / Cingeľová Maruščáková, IvanaSchusterová, PetraPopelka, PeterGancarčíková, SoňaCsank, TomášFečkaninová, AdriánaRatvaj, MarekMudroňová, Dagmar. In: Fish & shellfish immunology, 2021, 119, 379-383.

Basic data
OCA1. Surname awarded to the assessed person	Popelka
OCA2. Name awarded to the assessed person	Peter
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6062
OCA5. Area of assessment	study programme nutrition of animals and dietetics · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	431183
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=117E8DF36B6BB49F0650DF69F7
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Effect of autochthonous lactobacilli on immunologically important molecules of rainbow trout after bacterial infection studied on intestinal primoculture / Cingeľová Maruščáková, IvanaSchusterová, PetraPopelka, PeterGancarčíková, SoňaCsank, TomášFečkaninová, AdriánaRatvaj, MarekMudroňová, Dagmar. In: Fish & shellfish immunology, 2021, 119, 379-383.
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.sciencedirect.com/science/article/pii/S1050464821003454
OCA14. Characteristics of the author's contribution	The author is a co-creator of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Nowadays, the aquaculture industry is one of the fastest growing industries. Intensive aquaculture has a negative impact on fish health. Probiotic bacteria are often used due to beneficial effect to health of host, e.i. decrease of diseases outbreaks, immunomodulatory effect or better utilization of feed. The aim of this work was to study the influence of probiotic bacteria on the immune response of trout intestinal cells in primoculture infected with pathogenic bacteria. In the experiment, we tested the effect of pre-treatment of intestinal cells with an autochthonous strain of Lactobacillus plantarum R2 Biocenol™ (CCM 8674) following infection with the most serious salmonid pathogens – Aeromonas salmonicida subsp. salmonicida (CCM 1307) and Yersinia ruckeri (CCM 6093). Tested probiotic strain reduced inflammation after A. salmonicida infection through decreased expression of pro-inflammatory cytokines and increased expression of anti-inflammatory cytokines. In contrast, after infection with Y. ruckeri, which causes immunosuppression, the probiotic strain stimulated immunity by up-regulation of expression of proinflammatory cytokines and suppressed the expression of anti-inflammatory cytokines. These results are a prerequisite for the immunomodulatory potential of the strain, but its action must be confirmed in subsequent in vivo experiments.
Other data
OCA16. Annotation of the output in English	In this work, we standardized the preparation of primary culture from the rainbow trout intestine. The results of treatment of intestinal primary culture with autochthonous L. plantarum R2 and its products and subsequent infection with A. salmonicida or Y. ruckeri showed that the strain R2, as well as its products, reduced the expression of pro-inflammatory cytokines after A. salmonicida infection and, conversely, stimulated the expression of pro-inflammatory cytokine TNF-α and down-regulated anti-inflammatory IL-10 after treatment with the immunosuppressive Y. ruckeri. These findings indicate the immunomodulatory potential of the strain. This experiment was a pilot experiment before planned in vivo experiments on salmonid fish – Atlantic salmon and rainbow trout.
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	The result provides new insights into the use of probiotic bacteria in salmonid fish nutrition. Currently, aquaculture is one of the fastest growing industries. Intensive aquaculture has a negative impact on fish health. Probiotic bacteria are often used because of their beneficial effect on host health, e.g. reduction of disease incidence, immunomodulatory effect or improved feed utilization. The aim was to investigate the effect of probiotic bacteria on the immune response of intestinal cells of trout in primary culture infected with pathogenic bacteria.
OCA19. Characteristics of the output and related activities' impact on the educational process	The outputs can be used in animal nutrition education as well as microbiology and immunology.