SPIŠÁKOVÁ, Daniela [40 %] - KOŽÁROVÁ, Ivona [40 %] - HRICIKOVÁ, Simona [15 %] - MARCINČÁK, Slavomír [5 %]. Comprehensive screening of Salinomycin in feed and its residues in poultry tissues using microbial inhibition tests coupled to enzyme-linked immunosorbent assay (ELISA). In Foods. ISSN 2304-8158, 2024, vol. 13, no. 11, art. no. 1661, p. [1-17]. Dostupné na internete: .

Basic data
OCA1. Surname awarded to the assessed person	Kožárová
OCA2. Name awarded to the assessed person	Ivona
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6056
OCA5. Area of assessment	study programme food hygiene · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 1176745
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=2347834E6FE8D78CF315439ACDB8
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:001245394000001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	SPIŠÁKOVÁ, Daniela [40 %] - KOŽÁROVÁ, Ivona [40 %] - HRICIKOVÁ, Simona [15 %] - MARCINČÁK, Slavomír [5 %]. Comprehensive screening of Salinomycin in feed and its residues in poultry tissues using microbial inhibition tests coupled to enzyme-linked immunosorbent assay (ELISA). In Foods. ISSN 2304-8158, 2024, vol. 13, no. 11, art. no. 1661, p. [1-17]. Dostupné na internete: .
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2304-8158/13/11/1661
OCA14. Characteristics of the author's contribution	The author is the bearer of the main ideas of the scientific work of the postgraduate student (Spišáková D.) and the corresponding author of the scientific output. She is also the bearer of funds for the project from which the scientific output was realized.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Salinomycin is a coccidiostat approved as a feed additive for the prevention of coccidiosis in poultry. Official control of its residues is set by the Commission Delegated Regulation (EU) 2022/1644. The aim of our study was to assess the suitability of three microbial inhibition tests (MITs), Premi (R) Test, Explorer 2.0, and the Screening Test for Antibiotic Residues (STAR) linked to the enzyme-linked immunosorbent assay (ELISA), for the screening of salinomycin residues in the tissues of broiler chickens (breast and thigh muscle, heart, liver, gizzard, kidneys, lungs, spleen, skin, and fat) fed commercially produced feed containing 70 mg.kg-1 of salinomycin in the complete feed. The first residue screening (Sampling A) was performed on the last day of administration of the salinomycin-medicated feed (day 30), and the second screening (Sampling B) was performed on the day of slaughter (day 37) after the expiry of the withdrawal period with the feeding of non-medicated feed. Based on the quantitative confirmation of salinomycin residues in the examined chicken tissues by the ELISA method (Sampling A from 0.025 to 0.241 mg.kg-1; Sampling B from 0.003 to 0.076 mg.kg-1), all the MITs with the preference of the bacterial strain Bacillus stearothermophilus var. calidolactis ATCC 10149 demonstrated the ability to detect the residues of salinomycin in the examined tissues of broiler chickens at the level of the maximum residue limits set from 0.015 to 0.150 mg.kg-1 by Commission Implementing Regulation (EU) 2017/1914 and confirmed the relevance of their sensitivity to the coccidiostat salinomycin.
Other data
OCA16. Annotation of the output in English	Screening of coccidiostats in feed intended for poultry and their residues in poultry tissues is very important for the protection of public health and the guarantee of food safety. All three screening methods detected positive results and indicated the presence of salinomycin and its residues in the examined chicken tissues. The result of the screening methods was finally confirmed and quantitatively evaluated by the ELISA test. The connection between screening tests and the ELISA method clearly represents an effective tool for determining the residues of coccidiostats in poultry tissues and confirming the suitability of using the STAR method and screening tests with a preference for the Explorer 2.0 test for the relevant screening of coccidiostats and their residues within the initial screening of residues of antimicrobial substances in the tissues of food animals. Microbial inhibition tests are, so far, the only choice for the initial screening of antibiotic residues and can also be used for routine screening of coccidiostats and their residues.
OCA17. List of maximum 5 most significant citations corresponding to the output	1. HAMDI, Samiha - MÍGUEZ-GONZÁLEZ, Ainoa - CELA-DABLANCA, Raquel - BARREIRO, Ana - FERNÁNDEZ-SANJURJO, María J. - NÚÑEZ-DELGADO, Avelino - ÁLVAREZ-RODRÍGUEZ, Esperanza. Natural and modified clays as low-cost and ecofriendly materials to remove salinomycin from environmental compartments. In Journal of Environmental Management, 2024-09-01, 368, pp. ISSN 03014797. Dostupné na: https://doi.org/10.1016/j.jenvman.2024.122158., Registrované v: WOS, SCOPUS 2. LIU, Yuhong - SUN, Bolu - WU, Yuqiong - WANG, Hai - LI, Huajie - DANG, Qiaoning - WANG, Weijia - ZHOU, Miao - DA, Xuanxiu - HE, Haiying - KANG, Jiali - YANG, Lin - PU, Xiuying - MA, Quhuan. Fabricating electrochemical immunosensor based on magnetic multi-walled carbon nanotubes for rapid detection of early warning marker of acute myocardial infarction-Mb. In Microchemical Journal, 2025-08-01, 215, pp. ISSN 0026265X. Dostupné na: https://doi.org/10.1016/j.microc.2025.114449., Registrované v: WOS, SCOPUS 3. GODA, Mohamed N. - ALQARNI, Laila S. - IBRAHIM, Hossieny - EL-WEKIL, Mohamed M. - ALI, Al-Montaser Bellah H. Ratiometric fluorescence sensor for sensitive quantification of salinomycin residues in edible poultry tissues using R/CDs@Rh6G: A novel food safety approach. In MICROCHEMICAL JOURNAL, 2025, vol. 217, no., pp. ISSN 0026-265X. Dostupné na: https://doi.org/10.1016/j.microc.2025.114876., Registrované v: WOS 4. Tariq, N., Abbas, R. Z., & Tahir, S. (2026). Unveiling the therapeutic applications of Streptomyces albus against bacterial, fungal, and parasitic infections in poultry. World\'s Poultry Science Journal, 1-19. 5. Wang, S. Y., Liu, Q. Y., Tan, A. L., & Liu, L. (2026). Edible Oil Adulteration Analysis via QPCA and PSO-LSSVR Based on 3D-FS. Processes, 14(2), 390.
OCA18. Characteristics of the output's impact on socio-economic practice	The connection between screening tests and the ELISA method clearly represents an effective tool for determining the residues of coccidiostats in poultry tissues and confirming the suitability of using the STAR method and screening tests with a preference for the Explorer 2.0 test for the relevant screening of coccidiostats and their residues within the initial screening of residues of antimicrobial substances in the tissues of food animals. Microbial inhibition tests are, so far, the only choice for the initial screening of antibiotic residues and can also be used for routine screening of coccidiostats and their residues.
OCA19. Characteristics of the output and related activities' impact on the educational process	The outputs of the scientific article will be used in the implementation of scientific research activities of postgraduate education of doctoral students and as educational material presenting the legislation on official control of residues in products of animal origin.