· UVLF

Basic data
OCA1. Surname awarded to the assessed person	Popelka
OCA2. Name awarded to the assessed person	Peter
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6062
OCA5. Area of assessment	study programme nutrition of animals and dietetics · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2023
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	1057742
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=8BCE8CAA360877AE7D435FD0C94B
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.mdpi.com/2076-2615/13/12/1892
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)
OCA14. Characteristics of the author's contribution	Conceptualization and investigation.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The author is the originator of the original research idea and has been involved in research activities related to fieldwork, sampling and animal welfare during transport and killing.
Other data
OCA16. Annotation of the output in English	Intensive fish farming is associated with a high level of stress, causing immunosuppression. Immunomodulators of natural origin, such as probiotics or phytoadditives, represent a promising alternative for increasing the immune function of fish. In this study, we tested the autochthonous trout probiotic strain L. plantarum R2 in a newly developed, low-cost application form ensuring the rapid revitalization of bacteria. We tested continuous and cyclic feeding regimes with regard to their effect on the intestinal immune response and microbiota of rainbow trout. We found that during the continuous application of probiotic feed, the immune system adapts to the immunomodulator and there is no substantial stimulation of the intestinal immune response. During the cyclic treatment, after a 3-week break in probiotic feeding and the reintroduction of probiotics, there was a significant stimulation of the gene expression of molecules associated with both cellular and humoral immunity (CD8, TGF-β, IL8, TLR9), without affecting the gene expression for IL1 and TNF-α. We can conclude that, in aquaculture, this probiotic feed can be used with a continuous application, which does not cause excessive immunostimulation, or with a cyclic application, which provides the opportunity to stimulate the immunity of trout, for example, in periods of stress.
OCA17. List of maximum 5 most significant citations corresponding to the output	Junior K.S.C., Barros F.A.L., Do Couto M.V.S., Reis R.G.A., Marques M.H.C., Fujimoto R.Y., Cordeiro C.A.M., Da Costa Sousa N. AUTHOR FULL NAMES: Junior, Keber Santos Costa (58613942500); Barros, Francisco Alex Lima (57221109440); Do Couto, Marcia Valéria Silva (55856959200); Reis, Ryuller Gama Abreu (57191486221); Marques, Matheus Henrique Correa (57211537296); Fujimoto, Rodrigo Yudi (7101738519); Cordeiro, Carlos Alberto Martins (7006637645); Da Costa Sousa, Natalino (57191492225) 58613942500; 57221109440; 55856959200; 57191486221; 57211537296; 7101738519; 7006637645; 57191492225 Effects of an enterococcus faecium-based probiotic on growth performance and resistance of Amazon ornamental fish heros severus under short-term fasting [Efeitos de um probiótico à base de Enterococcus faecium no desempenho de crescimento e na resistência do peixe ornamental da Amazônia Heros severus em jejum de curta duração] (2025) Boletim do Instituto de Pesca, 51, art. no. e892 DOI: 10.20950/1678-2305/bip.2024.51.e892
OCA18. Characteristics of the output's impact on socio-economic practice	Output has impact on the use of probiotic bacteria in the aquaculture of salmonids, which are very sensitive to stress, represents a possibility to increase their resistance to diseases. We have developed a new, low-cost application form ensuring the rapid revitalization of probiotic bacteria. We tested continuous and cyclic feeding regimes with regard to their effect on the intestinal immune response and microbiota of rainbow trout. We found that continuous application stabilizes the intestinal microbiota in favor of beneficial lactic acid bacteria and does not cause unnecessary excessive stimulation of immunity, which can lead to an unwanted increase in energy demand. Cyclic application of probiotic feed has the same positive effect on the intestinal microbiota but provides the opportunity to stimulate the intestinal immune system of trout, for example, in periods of increased stress.
OCA19. Characteristics of the output and related activities' impact on the educational process	The results of the outcome have implications for education at all levels of the university in subjects focusing on animal nutrition and dietetics and aquatic animal microbiology and immunology.