ADC TKÁČIKOVÁ, Ľudmila - MOCHNÁČOVÁ, Evelína - PUNIT, Tyagi - KIŠŠOVÁ, Zuzana - BHIDE, Mangesh Ramesh. Comprehensive mapping of the cell response to E. coli infection in porcine intestinal epithelial cells pretreated with exopolysaccharide derived from Lactobacillus reuteri. In Veterinary Research. ISSN 0928-4249, 2020, vol. 51, no. 1, art. no. 49, p. [1-13]. (2019: 3.357 - IF, 3.432 - IF 5y, 99 - H-index, 1.425 - SJR, Q1 - SJR Best Q, Q1

ADC TKÁČIKOVÁ, Ľudmila - MOCHNÁČOVÁ, Evelína - PUNIT, Tyagi - KIŠŠOVÁ, Zuzana - BHIDE, Mangesh Ramesh. Comprehensive mapping of the cell response to E. coli infection in porcine intestinal epithelial cells pretreated with exopolysaccharide derived from Lactobacillus reuteri. In Veterinary Research. ISSN 0928-4249, 2020, vol. 51, no. 1, art. no. 49, p. [1-13]. (2019: 3.357 - IF, 3.432 - IF 5y, 99 - H-index, 1.425 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q).

Basic data
OCA1. Surname awarded to the assessed person	Tkáčiková
OCA2. Name awarded to the assessed person	Ľudmila
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/5991
OCA5. Area of assessment	study programme microbiology and immunology · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2020
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	182500
OCA9. Hyperlink to the record in CRPA or CRAA	https://arl4.library.sk/arl-uvlk/sk/detail-uvlk_un_cat-0034157-Comprehensi
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://arl4.library.sk/arl-uvlk/sk/detail-uvlk_un_cat-0034157-Comprehensive-mapping-of-the-cell-response-to-E-coli-infection-in-porcine-intestinal-epithelial-cel/?disprec=1&iset=7
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC TKÁČIKOVÁ, Ľudmila - MOCHNÁČOVÁ, Evelína - PUNIT, Tyagi - KIŠŠOVÁ, Zuzana - BHIDE, Mangesh Ramesh. Comprehensive mapping of the cell response to E. coli infection in porcine intestinal epithelial cells pretreated with exopolysaccharide derived from Lactobacillus reuteri. In Veterinary Research. ISSN 0928-4249, 2020, vol. 51, no. 1, art. no. 49, p. [1-13]. (2019: 3.357 - IF, 3.432 - IF 5y, 99 - H-index, 1.425 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q).
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://veterinaryresearch.biomedcentral.com/track/pdf/10.1186/s13567-020-00773-1.pdf
OCA14. Characteristics of the author's contribution	The author is the bearer of the main ideas and co-creator of the output. At the same time, it is also the holder of funds for the VEGA project from which the output was implemented.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Bacterial exopolysaccharides (EPSs) are known to modulate immunity. To date, a plethora of studies have reported the effect of EPSs on intestinal cells; however few works have revealed a complete picture of the signalling events in intestinal epithelial cells induced by bacterial EPSs. Here, using transcriptomics, we comprehensively mapped the biological processes in porcine intestinal epithelial cells challenged with EPS derived from Lactobacillus reuteri alone, enterotoxigenic Escherichia coli (ETEC) or ETEC after pretreatment with EPS. The Gene Ontology analysis of differentially expressed genes (DEGs) showed that ETEC is able to evoke biological processes specifically involved in cell junction reorganization, extracellular matrix degradation, and activation of the innate immune response through the activation of pattern recognition receptors, such as TLRs and CTRs. A total of 495 DEGs were induced in ETEC-challenged cells. On the other hand, EPS pretreatment was able to attenuate overexpression of the genes induced by ETEC infection.The most relevant finding of this study is that EPS has a suppressive effect on the inflammatory response evoked by ETEC infection. On the basis of high-throughput RNA-seq, this report is the first to describe the effects of EPSs derived from L. reuteri used as a pretreatment of global gene expression in porcine epithelial cells.
Other data
OCA16. Annotation of the output in English	Bacterial exopolysaccharides (EPSs) are known to modulate immunity. To date, a plethora of studies have reported the effect of EPSs on intestinal cells; however few works have revealed a complete picture of the signalling events in intes- tinal epithelial cells induced by bacterial EPSs. Here, using transcriptomics, we comprehensively mapped the biologi- cal processes in porcine intestinal epithelial cells challenged with EPS derived from Lactobacillus reuteri alone, entero- toxigenic Escherichia coli (ETEC) or ETEC after pretreatment with EPS. The Gene Ontology analysis of differentially expressed genes (DEGs) showed that ETEC is able to evoke biological processes specifically involved in cell junction reorganization, extracellular matrix degradation, and activation of the innate immune response through the activation of pattern recognition receptors, such as TLRs and CTRs. A total of 495 DEGs were induced in ETEC-challenged cells. On the other hand, EPS pretreatment was able to attenuate overexpression of the genes induced by ETEC infection. The most relevant finding of this study is that EPS has a suppressive effect on the inflammatory response evoked by ETEC infection. On the basis of high-throughput RNA-seq, this report is the first to describe the effects of EPSs derived from L. reuteri used as a pretreatment of global gene expression in porcine epithelial cells.
OCA17. List of maximum 5 most significant citations corresponding to the output	YANG, S. et al. Exopolysaccharides from lactic acid bacteria, as an alternative to antibiotics, on regulation of intestinal health and the immune system. In Animal Nutrition [online]. 2023, roč. 13, s. 78 – 89. Dostupné na: doi:10.1016/j.aninu.2023.02.004 YU, Z. et al. The role of potential probiotic strains Lactobacillus reuteri in various intestinal diseases: New roles for an old player. In Frontiers in Microbiology [online]. 2023, roč. 14. Dostupné na: doi:10.3389/fmicb.2023.1095555 WERNING, M.L. et al. Biological Functions of Exopolysaccharides from Lactic Acid Bacteria and Their Potential Benefits for Humans and Farmed Animals. In Foods [online]. 2022, roč. 11, č. 9. Dostupné na: doi:10.3390/foods11091284 ZAYED, A. et al. Rediscovering bacterial exopolysaccharides of terrestrial and marine origins: novel insights on their distribution, biosynthesis, biotechnological production, and future perspectives. In Critical Reviews in Biotechnology [online]. 2022, roč. 42, č. 4, s. 597 – 617. Dostupné na: doi:10.1080/07388551.2021.1942779 WU, Q. et al. Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells. In Frontiers in Veterinary Science [online]. 2021, roč. 8. Dostupné na: doi:10.3389/fvets.2021.677897
OCA18. Characteristics of the output's impact on socio-economic practice	The output brings new knowledge about the possibilities of using exopolysaccharides produced by probiotic bacteria Lactobacillus reuteri in influencing the inflammation of the gastrointestinal tract (GIT) of pigs caused by enterotoxigenic strains of E. coli.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output will be used to transmit knowledge in immunology, the study of the immune response in pigs\' gastrointestinal tracts (GIT).