A simple and rapid pipeline for identification of receptor-binding sites on the surface proteins of pathogens/ Patrícia Mertinková, Amod Kulkarni, Evelína Káňová, Katarína Bhide, Zuzana Tkáčová, Mangesh Ramesh Bhide. In Scientific Reports. - ISSN 2045-2322. - Vol. 10, no. (2020), art. no. 1163, p. [1-13]. (2019: 3.998 - IF, 4.576 - IF 5y, 179 - H-index, 1.341 - SJR, Q1 - SJR Best Q, Q1

A simple and rapid pipeline for identification of receptor-binding sites on the surface proteins of pathogens/ Patrícia Mertinková, Amod Kulkarni, Evelína Káňová, Katarína Bhide, Zuzana Tkáčová, Mangesh Ramesh Bhide. In Scientific Reports. - ISSN 2045-2322. - Vol. 10, no. (2020), art. no. 1163, p. [1-13]. (2019: 3.998 - IF, 4.576 - IF 5y, 179 - H-index, 1.341 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q).

Basic data
OCA1. Surname awarded to the assessed person	Bhide
OCA2. Name awarded to the assessed person	Mangesh Ramesh
OCA3. Degrees awarded to the assessed person	prof. MVSc., PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6102
OCA5. Area of assessment	study programme microbiology and immunology · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2020
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID = 177540
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildK174AQ&sid=5F1CDE603F7696E934F5F77A83&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/record/display.uri?eid=2-s2.0-85078248933&origin=resultslist&sort=plf-f&src=s&sid=ab9489fe123b1c014b295e00febce183&sot=b&sdt=b&sl=124&s=TITLE-ABS-KEY%28A+simple+and+rapid+pipeline+for+identification+of+receptor-binding+sites+on+the+surface+proteins+of+pathogens%29&relpos=0&citeCnt=1&searchTerm=&featureToggles=FEATURE_NEW_METRICS_SECTION:1
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	A simple and rapid pipeline for identification of receptor-binding sites on the surface proteins of pathogens/ Patrícia Mertinková, Amod Kulkarni, Evelína Káňová, Katarína Bhide, Zuzana Tkáčová, Mangesh Ramesh Bhide. In Scientific Reports. - ISSN 2045-2322. - Vol. 10, no. (2020), art. no. 1163, p. [1-13]. (2019: 3.998 - IF, 4.576 - IF 5y, 179 - H-index, 1.341 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q).
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.nature.com/articles/s41598-020-58305-y
OCA14. Characteristics of the author's contribution	The author is the bearer of ideas and the main researcher of all research in the field
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.
Other data
OCA16. Annotation of the output in English	Ligand-receptor interactions play a crucial role in the plethora of biological processes. Several methods have been established to reveal ligand-receptor interface, however, the majority of methods are time-consuming, laborious and expensive. Here we present a straightforward and simple pipeline to identify putative receptor-binding sites on the pathogen ligands. Two model ligands (bait proteins), domain III of protein E of West Nile virus and NadA of Neisseria meningitidis, were incubated with the proteins of human brain microvascular endothelial cells immobilized on nitrocellulose or PVDF membrane, the complex was trypsinized on-membrane, bound peptides of the bait proteins were recovered and detected on MALDI-TOF. Two peptides of DIII (~916 Da and ~2003 Da) and four peptides of NadA (~1453 Da, ~1810 Da, ~2051 Da and ~2433 Da) were identified as plausible receptor-binders. Further, binding of the identified peptides to the proteins of endothelial cells was corroborated using biotinylated synthetic analogues in ELISA and immunocytochemistry. Experimental pipeline presented here can be upscaled easily to map receptor-binding sites on several ligands simultaneously. The approach is rapid, cost-effective and less laborious. The proposed experimental pipeline could be a simpler alternative or complementary method to the existing techniques used to reveal amino-acids involved in the ligand-receptor interface
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	A rapid method of mapping receptor-ligand interactions, which may be a simpler alternative to existing techniques used to detect these interactions.
OCA19. Characteristics of the output and related activities' impact on the educational process	Understanding the interaction between the pathogen and the host that is necessary for the study of biomedical disciplines.