| Basic data |
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| OCA1. Surname awarded to the assessed person | Bhide |
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| OCA2. Name awarded to the assessed person | Mangesh Ramesh |
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| OCA3. Degrees awarded to the assessed person | prof. MVSc., PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6102 |
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| OCA5. Area of assessment | study programme microbiology and immunology · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2020 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | ID = 203871 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioFormChildU7GMB&sid=BEDB5CC6AC5331222198D0F4C4&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://www.scopus.com/record/display.uri?eid=2-s2.0-85083838639&origin=resultslist&sort=plf-f&src=s&sid=c3f7f2bbfc9303e9d5dac1dfe48e4d1e&sot=b&sdt=b&sl=126&s=TITLE-ABS-KEY%28Identification+of+the+proteins+of+Borrelia+garinii+interacting+with+human+brain+microvascular+endothelial+cells%29&relpos=0&citeCnt=2&searchTerm=&featureToggles=FEATURE_NEW_METRICS_SECTION:1 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | Identification of the proteins of Borrelia garinii interacting with human brain microvascular endothelial cells,
Tkáčová, Zuzana; Borszéková Pulzová, Lucia; Mochnáčová, Evelína; Jimenez Munguia, Irene; Bhide, Katarína; Mertinková, Patrícia; Majerová, Petra; Kulkarni, Amod; Kováč, Andrej; Bhide, Mangesh Ramesh. In Ticks and Tick-Borne Diseases. - ISSN 1877-959X. - Vol. 11, no. 4 (2020), art. no. 101451, p. [1-14]. (2019: 2.749 - IF, 2.896 - IF 5y, 39 - H-index, 1.182 - SJR, Q1 - SJR Best Q, Q2 - JCR Best Q). |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.sciencedirect.com/science/article/pii/S1877959X19304649 |
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| OCA14. Characteristics of the author's contribution | The author is the bearer of ideas and the main researcher of all research in the field |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | |
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| Other data |
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| OCA16. Annotation of the output in English | Lyme borreliosis is one of the major tick-borne diseases in Europe. Events of the translocation across the blood-brain barrier (BBB) involve multiple interactions between borrelial surface proteins and receptors on the brain microvascular endothelial cells (hBMECs). In this study, we aimed to identify proteins of Borrelia that interact with hBMECs. The surface proteome of live Borrelia (a neuroinvasive strain of B. garinii) was crosslinked with biotin prior to its incubation with hBMECs. The interacting proteins were recovered by affinity purification, followed by SWATH-MS. Twenty-four interacting candidates were grouped into outer membrane proteins (n = 12) and inner membrane proteins (n = 12) based on the subcellular location (LocateP). Other algorithms like TMHMM 2.0 and LipoP, ontology search and literature review were subsequently applied to each of the identified protein candidates to shortlist the most probable interactors. Six proteins namely, LysM domain protein, BESBP-5, Antigen S1, CRASP-1 (Bg071), Erp23 protein and Mlp family Lipoprotein were selected to produce recombinant forms and experimentally validate their interaction with hBMECs. We present here a high-throughput approach of generating a dataset of plausible borrelial ligands followed by a systematic bioinformatic pipeline to categorize the proteins for experimental validation. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | Bacterial extracellular dna promotes β-amyloid aggregation, Microorganisms 9(6),1301, 2021
Conserved lysine residues in decorin binding proteins of Borrelia garinii are critical in adhesion to human brain microvascular endothelial cells, Molecular Microbiology 115(6), pp. 1395-1409, 2021 |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The output reveals an interactoma between Borrelia and brain microvascular endothelial cells (hBMECs). Recognition of the interactions of the neuroinvasive bacterium Borrelia garinii with BMEC allows further development of targeted ligand-blocking therapeutic agents. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | / The output describes our procedure for the identification of the interactoma of neuroinvasive pathogens. The procedure includes methods of culturing hBMECs, Borrelia, proteome biotinilization, SWATH-MS, bioinformatics analysis of the obtained data, cloning process and preparation of recombinant proteins, as well as the use of antigenic methods in biological validation of interactions. The methodology of this article can be used in the educational process. It can also be a model for identifying the interactoma of other pathogens during further doctoral studies. |
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