V3 / ADF14 DANČOVÁ, Nikola - REGECOVÁ, Ivana - GREGOVÁ, Gabriela - KIRÁLY, Ján - SZABÓOVÁ, Tatiana. Detection of resistant Escherichia coli during meat processing in food establishments. In Folia veterinaria. ISSN 0015-5748, 2024, roč. 68, č. 2, s. 26-32.

Basic data
OCA1. Surname awarded to the assessed person	Gregová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6132
OCA5. Area of assessment	study programme feed and foodstuff safety · I. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 1187855
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=46EC41480997D7F108BBABC35E13
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/pages/publications/86000383585?origin=resultslist
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	V3 / ADF14 DANČOVÁ, Nikola - REGECOVÁ, Ivana - GREGOVÁ, Gabriela - KIRÁLY, Ján - SZABÓOVÁ, Tatiana. Detection of resistant Escherichia coli during meat processing in food establishments. In Folia veterinaria. ISSN 0015-5748, 2024, roč. 68, č. 2, s. 26-32.
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://reference-global.com/article/10.2478/fv-2024-0014
OCA14. Characteristics of the author's contribution	The author is the source of the main ideas and co-author of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	From a veterinary point of view, meat obtained from food-producing animals is also a potential reservoir for foodborne pathogens, including Escherichia coli. The prevalence and spread of antibiotic-resistant E. coli represent a threat to public health. The susceptibility of the investigated strains to antibiotics and their combinations was determined using the modified microdilution method. Dominant resistance was detected against ampicillin, tetracycline, cotrimoxazole, ampicillin + sulbactam, ciprofloxacin, and cefuroxime. Resistance to ertapenem, cefotaxime, tigecycline, and ceftazidime was also detected sporadically. A frequent mechanism responsible for resistance was the generation of incomplete fluoroquinolone resistance, induced by mutation and penicillinase, with low enzyme expression. Multidrug-resistant strains were also detected. By PCR, the tetA gene was confirmed in 68% of the isolates, and the tetB gene in 1 isolate. The prevalence of the qnrA gene was 29% among the examined isolates. Two isolates were found to carry the qnrS gene. The qnrB gene, as well as a combination of qnrA and qnrB, was detected in one isolate.
Other data
OCA16. Annotation of the output in English	Meat is an important food source, the nutritional composition of which, in conjunction with its desirable taste, positively affects human development and health. From a veterinary point of view, meat obtained from food-producing animals is also a potential reservoir for foodborne pathogens, including Escherichia coli. The prevalence and spread of antibiotic-resistant E. coli represent a threat to public health. For these reasons, the aim of this work was to identify the isolates obtained from food processing plants and to confirm the presence of E. coli species based on phenotypic and genotypic expression. The susceptibility of the investigated strains to antibiotics and their combinations was determined using the modified microdilution method. Dominant resistance was detected against ampilicin, tetracycline, cotrimoxazole, ampilicin + sulbactam, ciprofloxacin, and cefuroxime. Resistance to ertapenem, cefotaxime, tigecycline, and ceftazidime was also detected sporadically. Frequent mechanism responsible for resistance was generated an incomplete fluoroquinolone resistance induced by mutation and penicillinase with low enzyme expression. The occurrence of multidrug-resistant strains was also detected. Selected E. coli isolates were subjected to detection of genes encoding resistance to tetracyclines (tetA and tetB) and quinolones (qnrA, qnrB, and qnrS) by PCR. The tetA gene was confirmed in 68 % of the isolates, and the tetB gene in one isolate. The prevalence of the qnrA gene was found to be 29 % in the examined isolates. Two isolates showed the presence of the qnrS gene. The qnrB gene, as well as a combination of the qnrA and qnrB genes, was detected in one isolate.
OCA17. List of maximum 5 most significant citations corresponding to the output	Shaukat, Faryal, et al.
OCA18. Characteristics of the output's impact on socio-economic practice	Excessive use of antibiotics in veterinary practice and failure to comply with good manufacturing practices in animal husbandry hygiene are significant factors in the emergence and spread of antibiotic-resistant microorganisms in animal husbandry. The presented publication focuses on comparing antibiotic resistance in E. coli bacteria from a meat processing plant and from broiler poultry meat, which poses a threat to public health. They can also be a source of the spread of antibiotic-resistant microorganisms into the food chain.
OCA19. Characteristics of the output and related activities' impact on the educational process	Antimicrobial resistance represents a serious global threat to humans, animals, and the environment. The publication offers an overview of resistance to individual ATB in indicator bacteria from animal husbandry. As part of teaching hygiene in the animal husbandry environment and sanitation in the food industry to UVLF students, it is necessary to highlight the importance of prevention, sanitation, and hygiene in reducing bacterial antimicrobial resistance.