| Basic data |
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| OCA1. Surname awarded to the assessed person | Popelka |
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| OCA2. Name awarded to the assessed person | Peter |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6062 |
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| OCA5. Area of assessment | habilitation and inauguration procedure – food hygiene and the environment |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2024 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 1154864 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=366D98E0417739F52150B474A316 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://www.scopus.com/pages/publications/85185693488 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | V3 |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-024-04394-5 |
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| OCA14. Characteristics of the author's contribution | The author is the source of the main ideas, co-author of the output and fund carrier. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | DNA topoisomerases regulate conformational changes in DNA topology during normal cell growth, such as replication, transcription, recombination, and repair, and may be targeted for anticancer drugs. A DNA topology assay was used to investigate DNA-damaging/protective activities of extracts from Habanero Red (HR), Habanero Maya Red (HMR), Trinidad Moruga Scorpion (TMS), Jalapeno (J), Serrano pepper (SP), Habanero Red Savina (HRS), Bhut Jolokia (BJ), and Jamaica Rosso (JR) peppers, demonstrating their inhibitory effect on the relaxation of pBR by Topo I. DNA topoisomerase II (Topo II) is proven therapeutic target of anticancer drugs. Complete inhibition of Topo II was observed for samples TMS, HR, and HMR. Extracts J and SP had the lowest capsaicin and dihydrocapsaicin content compared to other peppers. HR, HMR, TMS, J, S, HRS, BJ, JR extracts showed the anticancer effect, examined by MTS and xCell assay on the in vitro culture of human colon carcinoma cell line HCT116. |
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| Other data |
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| OCA16. Annotation of the output in English | DNA topoisomerases regulate conformational changes in DNA topology during normal cell growth, such as replication, transcription, recombination, and repair, and may be targeted for anticancer drugs. A DNA topology assay was used to investigate DNA-damaging/protective activities of extracts from Habanero Red (HR), Habanero Maya Red (HMR), Trinidad Moruga Scorpion (TMS), Jalapeno (J), Serrano pepper (SP), Habanero Red Savina (HRS), Bhut Jolokia (BJ), and Jamaica Rosso (JR) peppers, demonstrating their inhibitory effect on the relaxation of pBR by Topo I. DNA topoisomerase II (Topo II) is proven therapeutic target of anticancer drugs. Complete inhibition of Topo II was observed for samples TMS, HR, and HMR. Extracts J and SP had the lowest capsaicin and dihydrocapsaicin content compared to other peppers. HR, HMR, TMS, J, S, HRS, BJ, JR extracts showed the anticancer effect, examined by MTS and xCell assay on the in vitro culture of human colon carcinoma cell line HCT116. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | Corral-Guerrero I.A., Martínez-Medina A.E., Alvarado-Mata L.Y., Chávez A.C.F., Muñoz-García R., Luévanos-Escareño M.P., Sosa-Martínez J.D., Castro-Alonso M.J., Nimmakayala P., Reddy U.K., Balagurusamy N. Capsaicin as a Microbiome Modulator: Metabolic Interactions and Implications for Host Health (2025) Metabolites, 15 (6), art. no. 372
El-Haggar E.F., El-Sayed S.M., Kholif A.M.M., Ibrahim O.A. The Protective Action of Cephalotyre (Ras) Cheese Supplemented with Jalapenos Red Pepper on Inflammation and Colon Cancer (2025) Letters in Applied NanoBioScience, 14 (2), art. no. 54
Fatima S.R., Ahmed D. RSM-based green extraction of valuable bioactives from chili peppers using choline chloride-urea deep eutectic solvent coupled with microwave heating (2025) Biomass Conversion and Biorefinery |
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| OCA18. Characteristics of the output's impact on socio-economic practice | Our pilot study pointed out for the first time the inhibitory effect of some chili extracts on Topo I and II activity, as well as the relationship of such extracts to the reduction of metabolic activity and proliferation of human colon cancer cell line HCT116. TMS pepper completely inhibited Topo I and thus is the best candidate for further investigation, suitable for medicinal treatments because their underlying mechanisms differ from those of Topo I inhibitors. In order to confirm the relationship between the inhibition of topoisomerase activity and the antitumor effect of chili extracts, it is necessary to test a wider range of tumor cells as well as the concentration and time scale of the action of such extracts. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The contribution discusses the inhibitory properties of chili pepper extracts and their use in human medicine. The findings from the article concerning the use of extracts will be used in teaching subjects related to the processing of plant-based foods. |
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