HUDEC, Erik - MUDROŇOVÁ, Dagmar - MARCINČÁK, Slavomír - BARTKOVSKÝ, Martin - MAKIŠ, Andrej - FALDYNA, Martin - RATVAJ, Marek - KARAFFOVÁ, Viera. The effect of Limosilactobacillus fermentum 2i3 and 0.6% addition of humic substances on production parameters and the immune system of broilers. In Poultry Science. ISSN 0032-5791, 2024, vol. 103, no. 8, art. no. 103884, p. [1-11].

Basic data
OCA1. Surname awarded to the assessed person	Mudroňová
OCA2. Name awarded to the assessed person	Dagmar
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6094
OCA5. Area of assessment	habilitation and inauguration procedure – microbiology and immunology
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	1182844
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=069BD78221CDCAB105A59EF36A04
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:001253569900001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	HUDEC, Erik - MUDROŇOVÁ, Dagmar - MARCINČÁK, Slavomír - BARTKOVSKÝ, Martin - MAKIŠ, Andrej - FALDYNA, Martin - RATVAJ, Marek - KARAFFOVÁ, Viera. The effect of Limosilactobacillus fermentum 2i3 and 0.6% addition of humic substances on production parameters and the immune system of broilers. In Poultry Science. ISSN 0032-5791, 2024, vol. 103, no. 8, art. no. 103884, p. [1-11].
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.sciencedirect.com/science/article/pii/S0032579124004632?via%3Dihub
OCA14. Characteristics of the author's contribution	The author is one of the bearers of the main idea of the output and at the same time the implementer and creator of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The widespread use of antibiotics in the poultry industry as growth promoters has led to the emergence of bacterial resistance, which poses a significant health risk to humans and animals. Substances of natural origin, such as probiotic bacteria and humic substances, can be a promising solution. The aim of this experiment was to study the effect of the administration of a probiotic strain of Limosilactobacillus fermentum 2i3 and/or a new formula of humic substances specifically designed for detoxification on the production parameters, including gene expression of myogenic growth factors and selected parameters of the immune response of broilers. Based on the results obtained, we can confirm the immunostimulating effect of L. fermentum 2i3 administered in drinking water, which also had an impact on important production parameters of broiler meat. On the other hand, in the combined group there was no expected potentiation of the positive effects on the observed parameters.
Other data
OCA16. Annotation of the output in English	It is believed that probiotic bacteria, as well as HS, regularly show positive effects on health, but their direct impact on some production indicators are often contradictory. The aim of the trial was to study the effect of the administration of a probiotic strain of Limosilactobacillus fermentum 2i3 alone, and in combination with a new formula of humic substances specifically designed for detoxification. We monitored production parameters, including gene expression of myogenic growth factors and selected parameters of the immune response of broilers. Based on our obtained findings, we may confirm the immunostimulating effect of L. fermentum 2i3 administered in drinking water, which also had an impact on important production parameters of broilers. The application of a new formula of 0.6% humic substances significantly increased the relative gene expression for all myogenic growth factors, but showed no effect on production parameters. Moreover, it had a negative effect on phagocytic activity. However, the combination of probiotic strain with humic substances did not significantly affect the monitored parameters. We can conclude that the combination of HS and probiotics does not produce the desired potentiation of the effect on the immune response or on the production parameters of broilers.
OCA17. List of maximum 5 most significant citations corresponding to the output	Dančová N., Gregová G., Szabóová T., Marcinčák S. The effects of natural additives on litter condition, microclimate environment and antimicrobial resistance in the broiler chickens rearing, Frontiers in Sustainable Food Systems, 2025, 9, 2025, https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2025.1380876, DOI=10.3389/fsufs.2025.1380876 Herich, R.; Szabóová, R.; Karaffová, V.; Racines, M.P.; Šefcová, M.A.; Larrea-Álvarez, M. A Narrative Review on the Impact of Probiotic Supplementation on Muscle Development, Metabolic Regulation, and Fiber Traits Related to Meat Quality in Broiler Chickens. Microorganisms 2025, 13, 784. https://doi.org/10.3390/microorganisms13040784 D’Carmo Sodré MM, Araújo Cruz ID, Santos UR, Potente Dutra Luquetti SC, Silva VL, Ferreira Machado AB, Galuppo Diniz C, de Souza CT, Romano C, Marin LJ and de Carvalho LD (2026) Probiotic cell-free supernatants as a strategy against antimicrobial resistance: a systematic review. Front. Cell. Infect. Microbiol. 16:1731341. doi: 10.3389/fcimb.2026.1731341 Hreško Šamudovská, A., Karaffová, V., Marcin, A., Mihok, T., Harčárová, M., Koščová, J., … Bujňák, L. (2025). Effect of humic substances, Limosilactobacillus fermentum and their combinations on growth performance, fermentation activity of the microbial population and mucosal immunity in the digestive tract of turkeys. Italian Journal of Animal Science, 24(1), 1323–1335. https://doi.org/10.1080/1828051X.2025.2513389
OCA18. Characteristics of the output's impact on socio-economic practice	Based on this and our previous studies, where we applied HS to poultry, we can conclude that their effect on the immune response depends not only on the dose and source used, but also on the processing technology, which affects the size and structure of the humic acid molecules. Very large humic acid molecules with high absorption capacity appear to have much lower immunostimulatory abilities compared to smaller molecules. It means that this study brings knowledge about the influence of the technology used to process humic substance on important production and immunological indicators, which can be used for specific purposes in poultry farms. A very important practical finding is that the combination of HS and probiotics does not produce a potentiating effect; on the contrary, the effectiveness of the probiotics is reduced.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output brings new knowledge about the effect of humic substances alone, as well as their combination with probiotics, on the immune response of poultry. The doctoral students (Hudec, E., Makiš, A.) who worked on this research significantly expanded their methodological and theoretical background through the implementation of the experiments, and the obtained results are/will be part of their dissertations. The acquired knowledge will also be further used for teaching students in the third level of university studies in the program: Microbiology and Immunology.