ADC Effect of Cladosporium cladosporioides on the composition of mycoflora and the quality parameters of table eggs during storage / Jevinová Pavlina, Pipová Monika, Regecová Ivana, Demjanová Soňa, Semjon Boris, Marcinčák Slavomír, Nagy Jozef, Kožárová Ivona. In: Processes. -ISSN 2227-9717. -Vol. 9, no. 4 (2021), art. no. 613, p. 1-17

Basic data
OCA1. Surname awarded to the assessed person	Pipová
OCA2. Name awarded to the assessed person	Monika
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, CSc.
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6030
OCA5. Area of assessment	study programme food hygiene · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	305986
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildIP9F1&sid=26D3F01130691A98F974E8D10E&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000643607400001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Effect of Cladosporium cladosporioides on the composition of mycoflora and the quality parameters of table eggs during storage / Jevinová Pavlina, Pipová Monika, Regecová Ivana, Demjanová Soňa, Semjon Boris, Marcinčák Slavomír, Nagy Jozef, Kožárová Ivona. In: Processes. -ISSN 2227-9717. -Vol. 9, no. 4 (2021), art. no. 613, p. 1-17
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2227-9717/9/4/613
OCA14. Characteristics of the author's contribution	The author participated in the development of methodology, selection of suitable analytical methods, wring and editing. She also made all revisions of the manuscript according to the reviewers´recommendations, as well as final corrections of the output before its publication.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The experiment was performed with 120 one-day-old table eggs. Eggshells were contaminated with the spore suspension of Cladosporium cladosporioides, divided into three groups (A–C) and stored at three different temperatures commonly used for the storage of eggs in households (3 °C, 11 °C and 20 °C) for 28 days. Visible growth of molds on/in experimental eggs was not observed within the entire storage period. Three mold genera were identified using the PCR method. Noticeable changes in egg quality were observed in eggs stored at 20 °C, most of these eggs were downgraded at the end of storage period. The composition of egg mycoflora was greatly influenced by storage conditions. As demonstrated in this study, high relative air humidity in the egg storage room reduced the growth of C. cladosporioides, and favored the growth of Penicillium spp. Generally, Penicillium spp. and Fusarium spp. showed better growth intensity at increased water activity values (0.91–0.94). The results of this study have demonstrated that storage conditions are crucial for maintaining egg quality and mycological safety of table eggs for consumers. The most appropriate temperature for egg storage is 11 °C, and the optimum range of relative humidity lies between 75% and 81%.
Other data
OCA16. Annotation of the output in English	The eggshells of 120 one-day-old table eggs were contaminated with the spore suspension of Cladosporium cladosporioides, divided into three groups (A–C) and stored at three different temperatures (3 °C, 11 °C and 20 °C) for 28 days. Visible growth of molds on/in experimental eggs was not observed within the entire storage period. No significant differences in the numbers of molds were found between particular groups of eggs. However, the composition of egg mycoflora was greatly influenced by storage conditions. Three mold genera were identified using the PCR method. The highest mold numbers were determined on Day 14 (Groups A and C) and Day 21 (Group B) when the maximum relative humidity and dew point temperature were recorded. On the same days, the dominance of Penicillium spp. and the minimum eggshell firmness were observed. Noticeable changes in egg quality were observed during storage at 20 °C, and most of these eggs were downgraded at the end of storage period. The growth ability differed significantly among three mold genera. Penicillium spp. and Fusarium spp. showed better growth intensity at increased values (0.91–0.94) of water activity (aw) indicating a possible risk associated with the occurrence of mycotoxins in the egg contents.
OCA17. List of maximum 5 most significant citations corresponding to the output	ZDOLEC, Nevijo. Introduction to Special Issue: Study of Microbiological Safety in the Food Chain. In PROCESSES, 2022, vol. 10, no. 1, pp. Dostupné na: https://doi.org/10.3390/pr10010149. YANG, Jianlou - HAO, Zikai - ZHANG, Lantao - FU, Yuming - LIU, Hong. Surface fungal diversity and several mycotoxin-related genes’ expression profiles during the Lunar Palace 365 experiment. In Microbiome, 2022-12-01, 10, 1, pp. Dostupné na: https://doi.org/10.1186/s40168-022-01350-8. HOU, Anwei - LI, Bei - DENG, Zixin - XU, Min - DICKSCHAT, Jeroen S. Cladosporin, A Highly Potent Antimalaria Drug? In ChemBioChem, 2023-06-15, 24, 12, pp. ISSN 14394227. Dostupné na: https://doi.org/10.1002/cbic.202300154.
OCA18. Characteristics of the output's impact on socio-economic practice	Eggshell surface is regularly contaminated with high number of microorganimsms from the air and the ambient environment, including the spores of microscopic filamentous fungi. Storage under appropriate conditions (elevated temperature and relative air humidity) may result in qualitative changes, egg devaluation and significant economic losses. The presence of toxicogenic mould spieces of both Penicillium sp. and Fusarium sp. also poses the risk of possible production of toxic metabolites (mycotoxins) making the eggs unfit for human consumption.
OCA19. Characteristics of the output and related activities' impact on the educational process	Accurate species identification of microscopic filamentous fungi is part of the compulsory subject \"Food Microbiology and Foodborne Diseases\" because it is of great importance from the viewpoint of food safety.