ADC Antimicrobial potential of Lactobacillus plantarum strains isolated from Slovak raw sheep milk cheeses / Vataščinová Tatiana, Pipová, Monika, Fraqueza, Maria Joao, Maľa Pavel, Dudriková Eva, Drážovská Monika, Lauková Andrea. In: Journal of Dairy Science, -ISSN 1525-3198. - Vol. 103, no. 8 (2020), p. 6900-6903

Basic data
OCA1. Surname awarded to the assessed person	Pipová
OCA2. Name awarded to the assessed person	Monika
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, CSc.
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6030
OCA5. Area of assessment	study programme food hygiene · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2020
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	202030
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildULA2N&sid=556388A0BD298FD9C3FBA63977&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000550190500016
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Antimicrobial potential of Lactobacillus plantarum strains isolated from Slovak raw sheep milk cheeses / Vataščinová Tatiana, Pipová, Monika, Fraqueza, Maria Joao, Maľa Pavel, Dudriková Eva, Drážovská Monika, Lauková Andrea. In: Journal of Dairy Science, -ISSN 1525-3198. - Vol. 103, no. 8 (2020), p. 6900-6903
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.sciencedirect.com/science/article/pii/S0022030220304720?via%3Dihub
OCA14. Characteristics of the author's contribution	The author prepared a conceptual plan, provided methodological guidance and professional supervision and participated in the selection of suitable analytical methods, writing and editing of the manuscript, as well as in all revisions and final correction of the text before publication.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Samples of raw milk sheep cheeses collected from 23 different regions of Eastern Slovakia were investigated for the presence of lactic acid bacteria by inoculation of MRS agar plates. Using both the genus- and species-specific PCR methods, 43 out of 72 strains were identified as Lactobacillus spp. and 10 strains were confirmed as Lb. plantarum. The whole bacterial cultures of Lb. plantarum strains were screened for antimicrobial activity against six selected foodborne pathogens using disc diffusion assay. All cultures exhibited visible antibacterial activity especially against Pseudomonas aeruginosa and Escherichia coli. Then, the cell-free neutralized supernatants and partially purified bacteriocins were prepared from the four Lb. plantarum strains that exhibited the best antibacterial potential and tested the same way.The presence of plnEF gene was revealed in six Lb. plantarum strains, that of plnD gene in three strains and both the plnA and plnC genes were found in two strains. The presence of both plnR and plnL genes was only detected in a single Lb. plantarum isolate. Based on the results of this study, four strains of Lb. plantarum appeared to be suitable candidates for further testing in the dairy manufacturing sector, particularly in the production of raw sheep milk products.
Other data
OCA16. Annotation of the output in English	The objective of this study was to screen Lactobacillus plantarum strains isolated from the traditional Slovak raw sheep milk cheese for their inhibitory potential. Seventy-two strains of lactobacilli were obtained by inoculation of MRS agar plates. Using the PCR method, 43 strains were identified as Lactobacillus spp., and 10 strains were confirmed as Lb. plantarum. First, the whole bacterial cultures of Lb. plantarum strains were tested by disc diffusion assay. All showed very good antibacterial activities against 6 selected foodborne pathogens, including Escherichia coli, Salmonella Enteritidis, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Then, cell-free neutralized supernatants and partially purified bacteriocins were prepared from the four Lb. plantarum strains that exhibited the best antibacterial potential, and they were tested the same way as the whole bacterial cultures. Seven of the 10 Lb. plantarum strains harbored the plnEF gene, 3 strains harbored the plnD gene, and 2 strains possessed both the plnA and plnC genes that encode the production of the respective plantaricins. Based on the results of this study, four strains of Lb. plantarum appeared to be suitable candidates for further testing in the dairy manufacturing sector, particularly in the production of raw sheep milk products.
OCA17. List of maximum 5 most significant citations corresponding to the output	YUE, Yue - HE, Zhujun - ZHOU, Yonghua - ROSS, R. Paul - STANTON, Catherine - ZHAO, Jianxin - ZHANG, Hao - YANG, Bo - CHEN, Wei. Lactobacillus plantarum relieves diarrhea caused by enterotoxin-producing Escherichia coli through inflammation modulation and gut microbiota regulation. In FOOD & FUNCTION. ISSN 2042-6496, 2020, vol. 11, no. 12, pp. 10362-10374 SOHN, Hyejin - CHANG, You Hyun - YUNE, Jong Hyeok - JEONG, Chang Hee - SHIN, Dong Min - KWON, Hyuk Cheol - KIM, Do Hyun - HONG, Sung Wook - HWANG, Hyelyeon - JEONG, Jong Youn - HAN, Sung Gu. Probiotic Properties of Lactiplantibacillus plantarum LB5 Isolated from Kimchi Based on Nitrate Reducing Capability. In FOODS, 2020, vol. 9, no. 12, Art. no. 1777; doi:10.3390/foods9121777. SCANO, Alessandra - BARBERIS, Antonio - ORRU, Germano - FAIS, Sara - PUGGIONI, Francesca - SPISSU, Ylenia - SERRA, Pier Andrea - D\'HALLEWIN, Guy. Pseudomonas aeruginosa as a Potential Contaminant of Packed Fresh-Cut Lettuce in a Controlled Atmosphere. The Role of Phenotypes muc(+)/muc(-). In BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY. ISSN 2069-5837, 2021, vol. 11, no. 2, pp. 8716-8724. HEGAB, Ola W. - ABDEL-LATIF, Eman F. - ZAKI, Hamdy M. B. A. - MOAWAD, Ashraf A. Fundamental role of Lactobacillus plantarum and inulin in improving safety and quality of Karish cheese. In OPEN VETERINARY JOURNAL. ISSN 2226-4485, 2021, vol. 11, no. 3, pp. 356-363. JEONG, Chang-Hee - SOHN, Hyejin - HWANG, Hyelyeon - LEE, Ho-Jae - KIM, Tae-Woon - KIM, Dong-Sub - KIM, Chun-Sung - HAN, Sung-Gu - HONG, Sung-Wook. Comparison of the Probiotic Potential between Lactiplantibacillus plantarum Isolated from Kimchi and Standard Probiotic Strains Isolated from Different Sources. In FOODS, 2021, vol. 10, no. 9, pp.
OCA18. Characteristics of the output's impact on socio-economic practice	This output demonstrates significant probiotic potential and protective effect of Lactobacillus plantarum strains isolated from raw milk sheep cheese manufactured in Eastern Slovakia and suggests their commercial exploitation in dairy industry, particularly in the production of traditional Slovak raw sheep milk products, in an attempt to ensure safety and eliminate health risks to the consumers resulting from a possible presence of pathogenic and toxicogenic microorganisms in the finished products.
OCA19. Characteristics of the output and related activities' impact on the educational process	The methods used to extract and purify bacteriocins from bacterial cultures of lactobacilli, as well as those used for testing their antibacterial activity can be demonstrated and directly implemented into educational process in the postgraduate study.