ADC Species identification and antimicrobial resistance of coagulase-negative staphylococci isolated from the meat of sea fish / Regecová Ivana, Pipová Monika, Jevinová Pavlína, Marušková Katarína, Kmeť Vladimír, Popelka Peter. In: Journal of Food Science. - ISSN 0022-1147. - Vol. 79, no. 5 (2014), p. M898-M902

Basic data
OCA1. Surname awarded to the assessed person	Pipová
OCA2. Name awarded to the assessed person	Monika
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, CSc.
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6030
OCA5. Area of assessment	study programme food hygiene · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2014
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	UVL.Košice.0020003
OCA9. Hyperlink to the record in CRPA or CRAA	http://www.crepc.sk/portal?fn=*recview&uid=1354141&pageId=resultform&full=0
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000335491600017
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Species identification and antimicrobial resistance of coagulase-negative staphylococci isolated from the meat of sea fish / Regecová Ivana, Pipová Monika, Jevinová Pavlína, Marušková Katarína, Kmeť Vladimír, Popelka Peter. In: Journal of Food Science. - ISSN 0022-1147. - Vol. 79, no. 5 (2014), p. M898-M902
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://onlinelibrary.wiley.com/doi/10.1111/1750-3841.12429
OCA14. Characteristics of the author's contribution	The author prepared a conceptual plan, provided methodological guidance and professional supervision and participated in the selection of suitable analytical methods, writing and editing of the manuscript, as well as in making all revisions and final corrections of the output before publication.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The study is focused on both species identification and determination of antimicrobial resistance in coagulase-negative staphylococci (ČoNS) isolated from the meat of Atlantic mackerel (Scomber scombrus), Alaska pollock (Theragra chalcogramma), and Atlantic herring (Clupea harengus). Seventy-eight isolates of Staphylococcus spp. were identified with the help of genus-specific PCR method. All isolates were coagulase-negative, and DNAse activity was only confirmed in four of them. Using MALDI-TOF mass spectrometry, the following species of staphylococci were confirmed: S. warneri (52%), S. epidermidis (33%), S. haemolyticus (6.4%), S. pasteuri (3.8%), S. sciuri (1.2%), S. capitis (1.2%), and S. hominis (1.2%). The results showed that MALDI-TOF mass spectrometry is useful, accurate, and rapid method for species identification of staphylococci. Resistance to seven antibiotics was determined with the help of agar dilution method. The rate of antimicrobial resistance differed significantly among species of staphylococci tested. Most strains showed resistance against ampicillin (87%) and susceptibility to gentamicin (96%). Resistance to cefoxitin was observed in only one S. warneri strain. Furthermore, 83% of staphylococcal isolates were simultaneously resistant to two or more antibiotics. Although CoNS in fish is not a food poisoning issue, fish may serve as indicators of the spread of antibiotic resistant bacteria/genes in the environment.
Other data
OCA16. Annotation of the output in English	Seventy-eight isolates of staphylococci obtained from the meat of Theragra chalcogramma, Scomber scombrus, and Clupea harengus were identified and tested in this study. 16S rDNA sequence specific for the genus Staphylococcus was detected in all isolates with the help of PCR method. All of 78 isolates were coagulase-negative, and DNAse activity was only confirmed in four of them. The following species of staphylococci were identified using MALDI-TOF mass spectrometry: S. warneri (52%), S. epidermidis (33%), S. haemolyticus (6.4%), S. pasteuri (3.8%), S. sciuri (1.2%), S. capitis (1.2%), and S. hominis (1.2%). Antimicrobial resistance to seven antibiotics was determined in each isolate with the help of agar dilution method. In general, resistance against ampicillin was observed in majority of isolates (87%). On the contrary, the best ssusceptibility was determined to gentamicin (96%). Only one S. warneri strain showed resistance to cefoxitin. Furthermore, 83% of staphylococcal isolates were simultaneously resistant to two or more antibiotics.
OCA17. List of maximum 5 most significant citations corresponding to the output	SERGELIDIS, D. - ABRAHIM, A. - PAPADOPOULOS, T. - SOULTOS, N. - MARTZIOU, E. - KOULOURIDA, V. - GOVARIS, A. - PEXARA, A. - ZDRAGAS, A. - PAPA, A. Isolation of methicillin-resistant Staphylococcus spp. from ready-to-eat fish products. In LETTERS IN APPLIED MICROBIOLOGY. ISSN 0266-8254, 2014, vol. 59, no. 5, pp. 500-506. MOURA, Ana T. - SARAIVA, Cristina - MIGUEIS, Samuel - ESTEVES, Alexandra - FONTES, Maria C. Antimicrobial susceptibility of coagulase-positive and coagulase-negative staphylococci in ready-to-eat sashimi. In JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY. ISSN 1049-8850, 2017, vol. 26, no. 1, pp. 95-102., STAVROPOULOU, D. A. - DE VUYST, L. - LEROY, F. Nonconventional starter cultures of coagulase-negative staphylococci to produce animal-derived fermented foods, a SWOT analysis. In JOURNAL OF APPLIED MICROBIOLOGY. ISSN 1364-5072, 2018, vol. 125, no. 6, pp. 1570-1586. VAIYAPURI, Murugadas - JOSEPH, Toms Cheriyath - RAO, Badireddy Madhusudana - LALITHA, Kuttanapilly Velayudhan - PRASAD, Mothadaka Mukteswar. Methicillin-resistant Staphylococcus aureus in Seafood: Prevalence, laboratory detection, clonal nature, and control in seafood chain. In JOURNAL OF FOOD SCIENCE. ISSN 0022-1147, 2019, vol. 84, no., 12, pp., 3341-3351. SALGUEIRO, Vanessa - MANAGEIRO, Vera - BANDARRA, Narcisa M. - REIS, Ligia - FERREIRA, Eugenia - CANICA, Manuela. Bacterial diversity and antibiotic susceptibility of Sparus aurata from aquaculture. In MICROORGANISMS, 2020, vol. 8 (9)., no. 1343; doi:10.3390/microorganisms8091343..
OCA18. Characteristics of the output's impact on socio-economic practice	The output highlights the need of monitoring antimicrobial resistance in coagulase-negative staphylococci in the meat of retail marine fish, such as mackerels and herrrings, which can serve as significant source of transferable genetic elements encoding resistance to antibiotics used for treatment of disease in humans and animals.. The results of this study confirmed that MALDI-TOF mass spectrometry can be successfuly used for the preliminary species identification of staphylococcii present in food.
OCA19. Characteristics of the output and related activities' impact on the educational process	The methods used to isolate and identify bacterial food isolates as well as to testing their susceptibility to antimicrobials can be demonstrated and directly implemented in the postgradual educational process.