ADC Effect of fungal gamma-linolenic acid and beta-carotene containing prefermented feed on immunity and gut of broiler chicken. Mudroňová, DagmarKaraffová, VieraKoščová, JanaBartkovský, MartinMarcinčáková, DanaPopelka, PeterKlempová, TatianaČertík, MilanMačanga, JánMarcinčák, Slavomír. In Poultry Science, 2018, Roč. 97, č. 12, s. 4211-4218

Basic data
OCA1. Surname awarded to the assessed person	Popelka
OCA2. Name awarded to the assessed person	Peter
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6062
OCA5. Area of assessment	study programme nutrition of animals and dietetics · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2018
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	101611
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=6AEB618394BDC70BB080A15259
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000456702200009
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Effect of fungal gamma-linolenic acid and beta-carotene containing prefermented feed on immunity and gut of broiler chicken. Mudroňová, DagmarKaraffová, VieraKoščová, JanaBartkovský, MartinMarcinčáková, DanaPopelka, PeterKlempová, TatianaČertík, MilanMačanga, JánMarcinčák, Slavomír. In Poultry Science, 2018, Roč. 97, č. 12, s. 4211-4218
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://reader.elsevier.com/reader/sd/pii/S0032579119302494?token=7B38761CDBE5D72A611126B0CC00230D7E75DF62A03C7E921FEFD9F76E01FA68E7519006557E2162DC43C3A8BBA5C688&originRegion=eu-west-1&originCreation=20220807094711
OCA14. Characteristics of the author's contribution	The author is a co-creator of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Prefermented cereals containing fu gal GLA and carotene represent a low-cost supplement for broiler diet having immunomodulatory (by stimulating cellular immune response and gut IgA expression) and gut-protecting (by increasing expression levels for mucin 2 and insulin like growth factor 2) effects on the chicken. Received results showed that the used concentration was optimal for achieving a positive influence on the gut microbiota and immunity of broilers.
Other data
OCA16. Annotation of the output in English	Gamma-linolenic acid (GLA) is a fatty acid from the ω-6 family. It is able to deliver a wide range of health benefits arising from its anti-inflammatory effects. An insufficient supply of GLA from agricultural and animal sources resulted in the development of a fermentation technique using lower filamentous fungi, which have the ability to accumulate high concentrations of GLA and beta-carotene during solid-state fermentation of cereals. The goal of this study was to observe the influence of the addition of prefermented cereal product, containing high amounts of GLA and beta-carotene, into the feed of broiler chickens on their immune status, and also the number of lactic acid bacteria and enterobacteria in gut content, which has never been studied before. Immunostimulation in the GLA group was manifested by a significant increase in the oxidative burst of phagocytes, CD4+CD8- lymphocytes in blood, and the CD4: CD8 ratio. Upregulation of gene expression for IgA in the GLA group indicates that the B-lymphocytes were stimulated at a local gut level. In the caecum, increased mRNA expression for mucin-2 and insulin-like growth factor was observed in the GLA group, which could contribute mainly to the protection of the intestinal mucosa and to better growth and regeneration of skeletal muscles. Improved immune activation and protection of the intestinal mucosa were subsequently reflected in a change of the microbial composition in gut contents; a significant reduction of enterobacteria occurred after GLA administration. We can conclude that prefermented cereals containing fungal GLA and beta-carotene represent a low-cost supplement for broiler diet having a beneficial health effect.
OCA17. List of maximum 5 most significant citations corresponding to the output	Dulf, F.V., Vodnar, D.C., Toşa, M.I., Dulf, E.-H. Simultaneous enrichment of grape pomace with γ-linolenic acid and carotenoids by solid-state fermentation with Zygomycetes fungi and antioxidant potential of the bioprocessed substrates (2020) Food Chemistry, 310, art. no. 125927. Tang, J., Wei, X., Li, Y., Jiang, L., Feng, T., Zhu, H., Li, M., Chen, G., Yu, X., Zhang, J., Zhang, X. Poplar bark lipids enhance mouse immunity by inducing T cell proliferation and differentiation (2020) Journal of Veterinary Medical Science, 82 (8), pp. 1187-1196. Hui, J., Li, L., Li, R., Wu, M., Yang, Y., Wang, J., Fan, Y., Zheng, X. Effects of supplementation with β-carotene on the growth performance and intestinal mucosal barriers in layer-type cockerels (2020) Animal Science Journal, 91 (1), art. no. e13344.
OCA18. Characteristics of the output's impact on socio-economic practice	The output brings new knowledge about the possibilities of using gamma linolenic acid obtained by fermentation of waste products of the food industry using cultivated fungi by solid phase fermentation. The use in the nutrition of broiler chickens provides a number of benefits for their health, especially in the area of anti-inflammatory effects in the gastrointestinal tract.
OCA19. Characteristics of the output and related activities' impact on the educational process	The contribution addresses the mechanism of action of gamma linolenic acid in broiler chickens and at the same time addresses the possibilities of its use in the nutrition of broilers kept in intensive conditions on large-scale farms in order to improve their health. The outputs of the article concerning the feed production for broiler chickens will be used in teaching the subject Legislation requirements for feed and food production.